ADDITION OF PENICILLAMINE, HYPOTAURINE AND EPINEPHRINE (PHE) OR BOVINE OVIDUCTAL EPITHELIAL-CELLS (BOEC) ALONE OR IN COMBINATION TO BOVINE IN-VITRO FERTILIZATION MEDIUM INCREASES THE SUBSEQUENT EMBRYO CLEAVAGE RATE
ADDITION OF PENICILLAMINE, HYPOTAURINE AND EPINEPHRINE (PHE) OR BOVINE OVIDUCTAL EPITHELIAL-CELLS (BOEC) ALONE OR IN COMBINATION TO BOVINE IN-VITRO FERTILIZATION MEDIUM INCREASES THE SUBSEQUENT EMBRYO CLEAVAGE RATE
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DOI：
10.1016/0093-691x(94)90178-l
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发表时间：
1994-02-01
期刊：
影响因子：
2.8
通讯作者：
RORIE, RW
中科院分区：
文献类型：
--
作者：
MILLER, GF;GLIEDT, DW;RORIE, RW
在1）单独的TALP培养基（对照）; 2）TALP + BOEC; 3）TALP + PHE;或4）TALP + BOEC和PHE中受精后，比较体外成熟牛卵母细胞的卵裂率。授精后45 h的总体卵裂率，处理2（52%）、3（55%）和4（66%）的胚胎高于处理1（32%）。处理2和3的卵母细胞卵裂率相似，但低于处理4。添加PHE或BOEC，单独或组合，受精培养基导致更多的胚胎在3-或4-细胞阶段比2-细胞阶段的受精后45小时。在与BOEC在M-199培养基中共培养5d后，处理1、2、3和4中分别有21、28、25和35%的卵裂胚胎发育到桑椹胚或囊胚期。处理1、2和3之间以及处理2和4之间的桑椹胚和囊胚发育率相似。在各处理中，在授精后45 h达到3-或4-细胞期的胚胎部分与各处理中继续在体外发育至桑椹胚或囊胚期的胚胎百分比之间的相关性为0.98。
The cleavage rate of in vitro-matured bovine oocytes was compared after fertilization in 1) TALP medium alone (control); 2) in TALP + BOEC; 3) in TALP + PHE; or 4) in TALP + BOEC and PHE. The overall cleavage rate at 45 h post insemination was greater for embryos in Treatments 2 (52%), 3 (55%) and 4 (66%) than for Treatment 1 (32%). The oocyte cleavage rates for Treatments 2 and 3 were similar, but were lower than that of Treatment 4. Addition of PHE or BOEC, alone or in combination, to the fertilization medium resulted in more embryos at the 3- or 4-cell stage than the 2-cell stage by 45 h post insemination. After 5 d of co-culture with BOEC in M-199 medium, 21, 28, 25 and 35% of the cleaved embryos in Treatments 1, 2, 3 and 4, respectively, developed to the morula or blastocyst stage. The rate of development to morulae and blastocysts was similar among Treatments 1, 2 and 3, and between Treatments 2 and 4. Across treatments, a correlation of 0.98 was noted between the portion of embryos that had reached the 3- or 4-cell stage by 45 h post insemination and the percentage of embryos in each treatment that continued to develop to the morula or blastocyst stage in vitro.