Genomic structure of the RNA polymerase II small subunit (hRPB14.4) locus (POLRF) and mapping to 22q13.1 by sequence identity.
Genomic structure of the RNA polymerase II small subunit (hRPB14.4) locus (POLRF) and mapping to 22q13.1 by sequence identity.
复制标题
RNA 聚合酶 II 小亚基 (hRPB14.4) 位点 (POLRF) 的基因组结构，并通过序列同一性映射到 22q13.1。
DOI：
10.1006/geno.1996.0312
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Blin,N
中科院分区：
文献类型：
--
作者：
Pusch,C;Wang,Z;Roe,B;Blin,N
DNA依赖性RNA聚合酶II与超过20种辅因子组合，控制三种已知DNA依赖性RNA聚合酶之间的选择性以及真核生物中转录起始、延伸和终止的效率，聚合酶II（EC 2.7. 7.8）、α-鹅膏蕈碱（5）。该酶的10至14个亚基在敏感酶中变化很大，是负责转录的前分子量（220至7 kDa）。相应的cDNA，tein编码基因。根据其来源，它由10至14个多肽组成，这些多肽先前已被克隆和表征（综述见（5））。迄今为止，已经分离出17 p13（POLR 2A）、4 q12这些亚基，并克隆了其编码cDNA（POLR 2B）、16 q13-q21（POLR 2C）、11 q32和19 p13（PO）。最大亚基（220 kDa; POLR 2A）LR 2 E）和19 q12（POLR 2 I）的基因座（1）。编码的cDNA是第一个被定位的（17 pter-p12;（6））; 14.4-kDa肽在C端显示出同源性，因为据报道，另外几个亚基基因与酵母聚合酶亚基中心的同源性更高，显示了不同染色体上的位点（4，11，16，17，ABC 23（2），但其基因组结构和定位有和19;总结在（1））。以前没有确定过。从我们目前的研究，在早期的研究中，一个小亚基（hRPB 14。4)现在看来，这是第一个与酿酒酵母聚合酶亚基同源的聚合酶II亚基被分配到人染色体22。随着RPB 6/RPO 26基因编码的ABC 23的不断增加，克隆了大量的表达序列标签，对HeLa细胞的cDNA进行了定位。这两种蛋白质都显示出与来自基因组拉链结构域的新序列的推定亮氨酸同源性（2），并且具有72%的保守残基，在它们的测序项目中将变得越来越有价值。C-末端区域。已发表的cDNA序列为546 bp。我们已经将hRPB 14的基因。4cDNA（HGMW-ap-
The DNA-dependent RNA polymerase II, in combinantion with an array of over 20 cofactors, controls the selectivity Among the three known DNA-dependent RNA polymerases and efficiency of transcription initiation, elongation, and terin eukaryotes, polymerase II (EC 2.7. 7.8), an a-amanitin-mination (5). The enzyme’s 10 to 14 subunits vary greatly in sensitive enzyme, is responsible for the transcription of pro-molecular mass (220 to 7 kDa). The corresponding cDNAs, tein-coding genes. Depending on its origin, it is composed ofwhich previously have been cloned and characterized, have 10 to 14 polypeptides (for review, see (5)). To date, several ofbeen mapped to various genomic loci: 17p13 (POLR2A), 4q12 these subunits have been isolated, and the encoding cDNAs(POLR2B), 16q13–q21 (POLR2C), 11q32 and 19p13 (PO-cloned. The locus for the largest subunit (220 kDa; POLR2A) LR2E), and 19q12 (POLR2I)(1). The cDNA coding for the was the first one to be mapped (17pter–p12;(6)); assignments 14.4-kDa peptide was shown to have homology in the C-terfor several additional subunit genes were reported more re-minal region to the Saccharomyces polymerase subunit cently, showing loci at various chromosomes (4, 11, 16, 17, ABC23 (2) but its genomic structure and localization have and 19; summarized in (1)). not been previously determined. From our present studies, In earlier studies, the cDNA of a small subunit (hRPB14. 4) it now appears that this is the first polymerase II subunit to with homology to a Saccharomyces cerevisiae polymerase sub-be assigned to human chromosome 22. With the ever-increasunit (ABC23, encoded by the RPB6/RPO26 gene) was cloned ing number of expressed sequence tags, mapping of cDNAs from HeLa cells. Both proteins display a putative leucine-by homology with new sequences derived from the genome zipper domain (2) and have 72% conserved residues in theirsequencing project will become increasingly valuable. C-terminal regions. The published cDNA sequence is 546 bp. We have placed the gene for hRPB14. 4 cDNA (HGMW-ap-