Comparative analysis of glutamate transporter expression in rat brain using differential double in situ hybridization
Comparative analysis of glutamate transporter expression in rat brain using differential double in situ hybridization
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DOI：
10.1007/s004290050161
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发表时间：
1998-07-01
期刊：
影响因子：
--
通讯作者：
Hediger, MA
中科院分区：
文献类型：
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作者：
Berger, UV;Hediger, MA
本研究使用敏感的非放射性原位杂交技术比较了大鼠大脑中三种谷氨酸转运蛋白 EAAC1、GLT1 和 GLAST 的 mRNA 表达模式。结果证实了 EAAC1 mRNA 的主要神经元定位、GLAST mRNA 的星形胶质细胞和室管膜定位以及 GLT1 mRNA 的星形胶质细胞和神经元定位。此外，我们使用一种新颖的差异双杂交方案证明，GLT1 mRNA在神经元中的存在比以前想象的更广泛，并且它涵盖了新皮质中的大多数神经元、嗅球外部丛状层中的神经元、前嗅核背侧和腹侧部分的神经元、前内侧丘脑核中的大多数神经元、海马中的CA3锥体神经元和神经元中的神经元。劣质橄榄。此外，我们还发现不同大脑区域中 GLT1 和 GLAST mRNA 的表达水平存在显着差异，表明它们的 mRNA 水平受到不同机制的调节。最后，对于 EAAC1，我们还证明了表达水平的广泛分布和显着的异质性。 EAAC1 由白质束中迄今为止未被识别的细胞群强烈表达，例如胼胝体、穹窿伞或前连合。此外，前脑和小脑大部分灰质区域的分散细胞群中存在强烈的 EAAC1 表达。这些结果提供了关于这三种谷氨酸转运蛋白的精确细胞定位及其在 mRNA 水平上的调节的更详细信息。
This study compares the mRNA expression pattern for the three glutamate transporters EAAC1, GLT1 and GLAST in rat brain, using a sensitive non-radioactive in situ hybridization technique. The results confirm the predominantly neuronal localization of EAAC1 mRNA, the astroglial and ependymal localization of GLAST mRNA and the astroglial and neuronal localization of GLT1 mRNA. Further, we demonstrate, using a novel differential double hybridization protocol, that the presence of GLT1 mRNA in neurons is more widespread than previously thought, and that it encompasses the majority of neurons in the neocortex, neurons in the external plexiform layer in the olfactory bulb, neurons in dorsal and ventral parts of the anterior olfactory nucleus, the majority of neurons in the anteromedial thalamic nuclei, the CA3 pyramidal neurons in the hippocampus and neurons in the inferior olive. In addition, we demonstrate marked variations in the expression levels of GLT1 and GLAST mRNAs in different brain areas, suggesting that their mRNA levels are regulated by different mechanisms. Finally, for EAAC1 we demonstrate also a widespread distribution and a marked heterogeneity in the expression levels. EAAC1 is strongly expressed by a heretofore unrecognized group of cells in white matter tracts such as the corpus callosum, fimbria-fornix or anterior commissure. Also, strong EAAC1 expression is present in groups of scattered cells in grey matter areas of much of the forebrain and the cerebellum. These results provide more detailed information about the precise cellular localization of these three glutamate transporters and their regulation at the mRNA level.