Deploying an in vitro gut model to assay the impact of a mannan-oligosaccharide prebiotic, Bio-Mos® on the Atlantic salmon ( Salmo salar ) gut microbiome
Deploying an in vitro gut model to assay the impact of a mannan-oligosaccharide prebiotic, Bio-Mos® on the Atlantic salmon ( Salmo salar ) gut microbiome
复制标题
采用体外肠道模型来分析甘露寡糖益生元 Bio-Mos® 对大西洋鲑鱼 (Salmo salar) 肠道微生物组的影响
DOI：
10.1101/2021.08.18.456921
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Kazlauskaite R
中科院分区：
文献类型：
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作者：
Kazlauskaite R
甘露寡糖（MOS）益生元作为免疫调节剂广泛应用于畜牧业，并可促进生长和肠道健康。它们的作用模式被认为是通过它们对宿主微生物群落和相关代谢的影响来介导的。Bio-Mos是目前用于农业饲料行业的市售益生元。为了评估Bio-Mos作为鲑鱼水产养殖中益生元生长促进剂的潜在用途，我们修改了已建立的大西洋鲑鱼体外肠道模型SalmoSim，以评估其对宿主微生物群落的影响。从成年养殖鲑鱼幽门盲囊隔室的生物学一式三份接种，在SalmoSim中稳定微生物群落，然后暴露于益生元20天，然后是8天的“洗出”期。饮食中包含MOS导致甲酸（p= 0.001），丙酸（p= 0.037）和异戊酸（p= 0.024）水平的显着增加，与几个，主要是厌氧微生物属（梭杆菌，Agarivorans，假交替单胞菌）的丰度增加。用16 S rDNA标记物进行的DNA元编码证实了微生物群落组成响应于MOS补充的显著变化，观察到产乳酸肉杆菌的增加。结合先前的体内研究，将增强的挥发性脂肪酸产生与MOS补充剂与宿主生长和性能联系起来，我们的数据表明Bio-Mos可能在鲑鱼生产中具有价值。此外，我们的数据强调了体外肠道模型在增强微生物组调节剂体内试验中的潜在作用。
Mannose-oligosaccharide (MOS) pre-biotics are widely deployed in animal agriculture as immunomodulators as well as to enhance growth and gut health. Their mode of action is thought to be mediated through their impact on host microbial communities and the associated metabolism. Bio-Mos is a commercially available pre-biotic currently used in the agri-feed industry. To assess Bio-Mos for potential use as a prebiotic growth promotor in salmonid aquaculture, we have modified an established Atlantic salmon in vitro gut model, SalmoSim, to evaluate its impact on the host microbial communities. Inoculated from biological triplicates of adult farmed salmon pyloric caeca compartments, the microbial communities were stabilised in SalmoSim followed by a twenty-day exposure to the prebiotic and in turn followed by an eight day ‘wash out’period. Dietary inclusion of MOS resulted in a significant increase in formate (p= 0.001), propionate (p= 0.037) and isovalerate (p= 0.024) levels, correlated with increased abundances of several, principally, anaerobic microbial genera (Fusobacterium, Agarivorans, Pseudoalteromonas). DNA metabarcoding with the 16S rDNA marker confirmed a significant shift in microbial community composition in response to MOS supplementation with observed increase in lactic acid producing Carnobacterium. In conjunction with previous in vivo studies linking enhanced volatile fatty acid production alongside MOS supplementation to host growth and performance, our data suggests that Bio-Mos may be of value in salmonid production. Furthermore, our data highlights the potential role of in vitro gut models to augment in vivo trials of microbiome modulators.