Diverse contribution of Col2a1-expressing cells to the craniofacial skeletal cell lineages.
Diverse contribution of Col2a1-expressing cells to the craniofacial skeletal cell lineages.
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DOI：
10.1111/ocr.12168
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发表时间：
2017-06
影响因子：
3.1
通讯作者：
Ono N
中科院分区：
文献类型：
--
作者：
Sakagami N;Ono W;Ono N
颅面部骨骼发育需要软骨内和膜内两种不同的成骨机制的精心协调。表达Col2a1的细胞在肢体的软骨内骨中包含与生长相关的骨骼前体细胞。本研究的目的是确定表达Col2a1的细胞对头面部骨骼细胞谱系的贡献。我们假设，表达Col2a1的祖细胞对与颅面发育相关的各种骨化模式有重要贡献。利用转Col2a1-cre基因和R26R-tdTomato报告等位基因的cre-loxP系统研究了表达Col2a1细胞的细胞命运。我们分析了颅面部骨骼复合体的三个不同位置，它们代表了独特的骨化机制：颅底、头盖骨和下颌突。Col2a1-cre始终标记颅底的大多数骨骼细胞。有趣的是，Col2a1-cre还标记了颅骨中的大量成骨细胞和缝合间充质细胞，以及潜在的瞬时软骨中的软骨细胞。在下颌骨中，COL2a1-cre仅在生长期标记软骨细胞和成骨细胞。COL2a1在颅底正常的软骨内骨形成中由骨系祖细胞表达。相反，颅面复合体的其他成骨机制以不同的方式利用Col2a1表达的细胞，从而Col2a1可能在骨骼谱系的更多分化或瞬时细胞类型中表达。
Craniofacial skeletal development requires deliberate coordination of two distinct mechanisms of endochondral and intramembranous ossification. Col2a1-expressing cells encompass growth-associated skeletal progenitors in endochondral bones of the limb. The objective of this study is to determine the contribution of Col2a1-expressing cells to the craniofacial skeletal cell lineages. We hypothesize that Col2a1-expressing progenitors significantly contribute to various modes of ossification associated with the craniofacial development. Cellular fates of Col2a1-expressing cells were studied based on a cre-loxP system using a Col2a1-cre transgene and an R26R-tdTomato reporter allele. We analyzed three distinct locations of the craniofacial skeletal complex representing unique ossification mechanisms; the cranial base, the calvaria and the mandibular condyle. Col2a1-cre consistently marked a majority of skeletal cells in the cranial base. Interestingly, Col2a1-cre also marked a large number of osteoblasts and suture mesenchymal cells in the calvaria, in addition to chondrocytes in the underlying transient cartilage. In the mandibular condyle, Col2a1-cre marked chondrocytes and osteoblasts only during the growth phase. Col2a1 is expressed by progenitors of the skeletal lineage in canonical endochondral bone formation occurring in the cranial base. In contrast, other ossification mechanisms of the craniofacial complex utilize Col2a1-expressing cells in a different manner, whereby Col2a1 may be expressed in more differentiated or transient cell types of the skeletal lineage.