CDNA CLONING AND EXPRESSION OF THE HUMAN HOMOLOG OF THE SEA-URCHIN FASCIN AND DROSOPHILA SINGED GENES WHICH ENCODES AN ACTIN-BUNDLING PROTEIN
CDNA CLONING AND EXPRESSION OF THE HUMAN HOMOLOG OF THE SEA-URCHIN FASCIN AND DROSOPHILA SINGED GENES WHICH ENCODES AN ACTIN-BUNDLING PROTEIN
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DOI：
10.1089/dna.1994.13.821
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发表时间：
1994-08-01
影响因子：
3.1
通讯作者：
GNARRA, JR
中科院分区：
文献类型：
--
作者：
DUH, FM;LATIF, F;GNARRA, JR
从人畸胎癌基因文库中分离到与海胆、发束蛋白和果蝇烧灼基因产物具有广泛序列同源性的克隆。人类同源基因被称为HSN，是一个单拷贝基因，通过荧光原位杂交定位于人类染色体7p22，预测编码一个493个氨基酸的产物，分子质量约为55,000。该蛋白在大小上类似于筋膜蛋白和烧灼蛋白，以及之前从HeLa细胞中纯化的55kD肌动蛋白捆绑蛋白。针对55-kD HeLa蛋白的单抗与细菌表达的HSN融合蛋白有反应，表明HSN基因可能编码55-kD蛋白。HSN mRNA在所分析的所有人体组织中都有不同程度的表达，在生长活跃的肾癌细胞系和激活的淋巴细胞中高表达，但在静止的淋巴细胞中不表达，这表明HSN在增殖中具有功能作用。Fasin家族与其他特征的肌动蛋白结合蛋白缺乏同源性，这些蛋白的高度进化保守性表明它们的肌动蛋白捆绑特性具有重要的功能。
cDNA clones having extensive sequence identity with the sea urchin fascin and the Drosophila singed gene products were isolated from a human teratocarcinoma cDNA library. The human homolog, termed hsn, is a single-copy gene that was localized to human chromosome 7p22 by fluorescence in situ hybridization and is predicted to encode a 493-amino-acid product with a molecular mass of approximately 55,000. This protein would be similar in size to the fascin and singed proteins, as well as a previously described 55-kD actin-bundling protein that was purified from HeLa cells. Monoclonal antibodies directed against the 55-kD HeLa protein were reactive against a bacterially expressed hsn fusion protein, indicating that the hsn gene probably encodes the 55-kD protein. The hsn mRNA was variably expressed in all human tissues analyzed and was highly expressed in actively growing renal carcinoma cell lines and in activated, but not in resting, lymphocytes, suggesting a functional role for hsn in proliferation. The fascin family lacks homology with other characterized actin-binding proteins, and the high degree of evolutionary conservation of these proteins indicates a functional importance of their actin-bundling properties.