Human herpesvirus 6 (HHV-6) is transmitted from parent to child in an integrated form and characterization of cases with chromosomally integrated HHV-6 DNA
Human herpesvirus 6 (HHV-6) is transmitted from parent to child in an integrated form and characterization of cases with chromosomally integrated HHV-6 DNA
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DOI：
10.1002/jmv.20113
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发表时间：
2004-07-01
影响因子：
12.7
通讯作者：
Yamanishi, K
中科院分区：
文献类型：
--
作者：
Tanaka-Taya, K;Sashihara, J;Yamanishi, K
我们从 2,332 名个体中获取了 7,566 份外周血单核细胞 (PBMC) 样本，并对他们进行了人类疱疹病毒感染筛查。我们确定了 5 个人的 PBMC 中持续含有高拷贝数人类疱疹病毒 6 (HHV-6) DNA。在这些个体的其他体细胞组织中也检测到了 HHV-6 DNA。他们的家庭成员中又发现了五例病例。对于其中两个家族，通过荧光原位杂交在 PBMC 中检测到染色体整合的 HHV-6 DNA (CIHHV-6)。所有受试者中CIHHV-6的患病率为0.21%。 HHV-6 DNA 在 4 个科中为 B 型，在 1 个科中为 A 型。在患有 CIHHV-6 的个体中分别检测到针对即刻早期抗原和糖蛋白 B 的抗体，在不患有 CIHHV-6 的健康志愿者中分别检测到 0% 和 60% 的抗体。使用 CIHHV-6 无法从 PBMC 中分离出 HHV-6。这些病例没有共同的临床特征，并且包括三名健康个体。我们的数据表明，CIHHV-6 很少见，但在普通人群中可以检测到，并且遗传传播是 HHV-6 传播途径之一。 (C) 2004 Wiley-Liss, Inc.
We obtained 7,566 peripheral blood mononuclear cell (PBMC) samples from 2,332 individuals and screened them for human herpesvirus infection. We identified five individuals who persistently harbored high copy numbers of human herpesvirus 6 (HHV-6) DNA in their PBMCs. HHV-6 DNA was also detected in other somatic tissues of these individuals. Five additional cases were identified among their family members. For two of these families, chromosomally integrated HHV-6 DNA (CIHHV-6) was detected in the PBMCs by fluorescence in situ hybridization. The prevalence of CIHHV-6 among all the subjects was 0.21%. The HHV-6 DNA was variant B in four families and variant A in one family. Antibodies to immediate early antigen and glycoprotein B were detected in 57 and 14% of individuals with CIHHV-6 and in 0 and 60% of healthy volunteers without CIHHV-6, respectively. HHV-6 could not be isolated from PBMCs with CIHHV-6. These cases shared no clinical features, and included three healthy individuals. Our data suggest that CIHHV-6 is rare but detectable in the general population and that hereditary transmission is one of the routes of HHV-6 transmission. (C) 2004 Wiley-Liss, Inc.