The importance of an endotoxin-free environment during the production of nanoparticles used in medical applications
The importance of an endotoxin-free environment during the production of nanoparticles used in medical applications
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DOI：
10.1021/nl060860z
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发表时间：
2006-08-09
期刊：
影响因子：
10.8
通讯作者：
Gabrielsson, Susanne
中科院分区：
文献类型：
--
作者：
Vallhov, Helen;Qin, Jian;Gabrielsson, Susanne
我们研究了球形金纳米颗粒对未成熟树突状细胞（DC）的影响。传统生产的纳米颗粒对 DC 具有成熟作用，这是脂多糖 (LPS) 污染的结果。通过改进生产工艺，获得了低LPS颗粒，该颗粒对DC的表型成熟或细胞因子的产生几乎没有影响。我们的研究结果强调了高纯度在纳米颗粒生产中的重要性，因为可能的污染物可能会干扰生物/医学效应的评估。他们还强调纳米颗粒可以作为免疫调节污染物的载体。
We investigated the effect of spherical gold nanoparticles on immature dendritic cells (DCs). Conventionally produced nanoparticles had a maturating effect on the DCs-a result of lipopolysaccharide (LPS) contamination. By modification of the production process, low-LPS particles were obtained, which had practically no effect on phenotypic maturation or cytokine production of the DCs. Our findings emphasize the importance of high purity in the production of nanoparticles, since possible contaminants may interfere with the assessment of biological/medical effects. They also highlight that nanoparticles can function as carriers of immune modulating contaminants.