Protective Effect of Donepezil on Retinal Ganglion Cells In Vitro and In Vivo
Protective Effect of Donepezil on Retinal Ganglion Cells In Vitro and In Vivo
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DOI：
10.1080/02713680500477438
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发表时间：
2006-01
影响因子：
2
通讯作者：
A. Miki;Y. Otori;T. Morimoto;M. Okada;Y. Tano
中科院分区：
文献类型：
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作者：
A. Miki;Y. Otori;T. Morimoto;M. Okada;Y. Tano
研究乙酰胆碱酯酶抑制剂多奈哌齐对视网膜神经节细胞（RGCs）的神经保护作用。在培养3天后，测量了暴露于含或不含多奈哌齐的谷氨酸盐（10 - 7 M至10 - 5 M）后纯化的RGC的存活率。在体内，多奈哌齐的神经保护作用通过定量成年大鼠轴突切断后7天存活的RGC的数量来检查。在体外，多奈哌齐剂量依赖性地减少谷氨酸毒性引起的RGC死亡。口服多奈哌齐（10 mg/kg-1天-1）可显著降低轴突切开术后RGC死亡。总之，多奈哌齐在体外和体内均对RGCs发挥保护作用。
The neuroprotective effect of donepezil, an acetylcholinesterase inhibitor on retinal ganglion cells (RGCs), was examined. The survival of purified RGCs after exposure to glutamate with or without donepezil (10− 7 M to 10− 5 M) was measured after 3 days in culture. In vivo, the neuroprotective effect of donepezil was examined by quantifying the number of viable RGCs 7 days after axotomy in adult rats. In vitro, donepezil dose-dependently reduced RGC death caused by glutamate toxicity. Oral administration of donepezil (10 mg kg−1 day−1) significantly reduced RGC death after axotomy. In conclusion, donepezil exerts a protective effect on RGCs both in vitro and in vivo.