Chitosan-alginate hybrid scaffolds for bone tissue engineering
Chitosan-alginate hybrid scaffolds for bone tissue engineering
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DOI：
10.1016/j.biomaterials.2004.09.062
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发表时间：
2005-06-01
期刊：
影响因子：
14
通讯作者：
Zhang, MQ
中科院分区：
文献类型：
--
作者：
Li, ZS;Ramay, HR;Zhang, MQ
组织工程中的生物可降解支架作为临时骨架来容纳和刺激新组织生长。在这里，我们报告的发展，由天然衍生的壳聚糖和海藻酸盐聚合物制成的生物可降解的多孔支架与其壳聚糖对应物相比，具有显着改善的机械和生物性能。增强的机械性能可归因于壳聚糖和海藻酸盐的复杂结构的形成。成骨细胞很容易附着在壳聚糖-藻酸盐支架上，增殖良好，并沉积钙化基质。体内研究表明，杂化支架具有高度的组织相容性。钙沉积早在植入后第4周发生。壳聚糖-藻酸盐支架可以由生理pH的溶液制备，这可以提供用于掺入蛋白质的有利环境，具有较小的变性风险。壳聚糖和藻酸盐的凝聚结合液-固分离提供了具有高孔隙率的支架，以及适合于快速进入临床试验的机械和生物学性质。(C)2004 Elsevier Ltd.保留所有权利。
A biodegradable scaffold in tissue engineering serves as a temporary skeleton to accommodate and stimulate new tissue growth. Here we report on the development of a biodegradable porous scaffold made from naturally derived chitosan and alginate polymers with significantly improved mechanical and biological properties as compared to its chitosan counterpart. Enhanced mechanical properties were attributable to the formation of a complex structure of chitosan and alginate. Bone-forming osteoblasts readily attached to the chitosan-alginate scaffold, proliferated well, and deposited calcified matrix. The in vivo study showed that the hybrid scaffold had a high degree of tissue compatibility. Calcium deposition occurred as early as the fourth week after implantation. The chitosan-alginate scaffold can be prepared from solutions of physiological pH, which may provide a favorable environment for incorporating proteins with less risk of denaturation. Coacervation of chitosan and alginate combined with liquid-solid separation provides a scaffold with high porosity, and mechanical and biological properties suitable for rapid advancement into clinical trials. (C) 2004 Elsevier Ltd. All rights reserved.