Reactive extraction of N-acetylneuraminic acid—Kinetic model and simulation of integrated product removal
Reactive extraction of N-acetylneuraminic acid—Kinetic model and simulation of integrated product removal
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DOI：
10.1016/j.seppur.2008.04.005
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发表时间：
2008-10
影响因子：
8.6
通讯作者：
V. Zimmermann;Ines Masuck;U. Kragl
中科院分区：
文献类型：
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作者：
V. Zimmermann;Ines Masuck;U. Kragl
苯硼酸（PBA）-三辛基甲基氯化铵（TOMAC）反应萃取N-乙酰神经氨酸是一种从反应液中回收神经氨酸的新方法。我们描述了一个动力学模型，这是适合于描述纯化合物以及反应物的混合物，这是N-乙酰基-D-葡糖胺（GlcNAc），N-乙酰基-D-甘露糖胺（ManNAc），丙酮酸钠（Pyr）和神经氨酸（Neu 5Ac）的提取。对于提取的两步机制被假定和界面处的离子交换被确定为限速步骤。在Lewis型电池中进行动力学测量。第一反应步骤通过描述碳水化合物的可提取量的参数σ包括在模型中。提取模型与描述从GlcNAc酶促合成Neu 5Ac的模型相结合，以模拟反应和提取的集成。集成过程中模拟了不同的基板浓度，载流子浓度，相比和界面面积。开发了一种间歇反应，同时萃取和连续更新的有机相。在优化的条件下，神经氨酸以与没有萃取相同的时空产率产生，并且产物积累在有机相中，这有利于进一步的下游加工。所建立的模型可用于分析和评价反应和萃取过程的一体化产物脱除和分离操作。
The reactive extraction of N-acetylneuraminic acid with phenylboronic acid (PBA) and trioctylmethylammoniumchloride (TOMAC) is a new method to recover neuraminic acid from reaction solutions. We describe a kinetic model which is suitable to describe the extraction of pure compounds as well as mixtures of the reactants, which are N-acetyl-d-glucosamine (GlcNAc), N-acetyl-d-mannosamine (ManNAc), sodium pyruvate (Pyr) and neuraminic acid (Neu5Ac). For the extraction a two-step mechanism is assumed and the ion exchange at the interface was determined to be the rate-limiting step. Kinetic measurements were performed in a Lewis-type cell. The first reaction step is included in the model by a parameter σ which describes the extractable amount of carbohydrate. The extraction model is combined with a model describing the enzymatic synthesis of Neu5Ac from GlcNAc to simulate the integration of reaction and extraction. The integrated process was simulated with varying substrate concentrations, carrier concentrations, phase ratios and interfacial area. A batch reaction with concurrent extraction and continuous renewal of the organic phase was developed. Under optimised conditions neuraminic acid is produced with the same space–time yield as without extraction and the product is accumulated in the organic phase, which facilitates further downstream processing. The developed model is feasible to analyse and evaluate integrated product removal as well as separately operated reaction and extraction.