Microalgae-based bioactive hydrogel loaded with quorum sensing inhibitor promotes infected wound healing
Microalgae-based bioactive hydrogel loaded with quorum sensing inhibitor promotes infected wound healing
复制标题
负载群体感应抑制剂的基于微藻的生物活性水凝胶促进感染伤口愈合
DOI：
10.1016/j.nantod.2021.101368
复制
发表时间：
2022-02-01
期刊：
影响因子：
17.4
通讯作者：
Zhou, Min
中科院分区：
文献类型：
--
作者：
Hu, Huiqun;Zhong, Danni;Zhou, Min
细菌物种之间的相互作用是通过群体感应（QS），这是一个调节和协调毒力活动和生物膜形成基因的过程。QS通过诱导伤口的慢性炎症，在伤口愈合过程中有助于减缓组织修复。在这里，我们开发了一种基于生物活性水凝胶系统的多功能QS抑制剂，以中断细菌的QS，缓解缺氧，破坏生物膜，从而加速糖尿病小鼠感染伤口的愈合。将小檗碱（BBR，一种QS抑制剂和抗菌剂）负载到天然活微藻钝顶螺旋藻（Spirulina platensis，SP）中，与羧甲基壳聚糖/海藻酸钠组合形成生物活性水凝胶（BBR@SP gel）。在激光照射下，BBR@SP凝胶可持续释放BBR并产生活性氧，从而协同QS抑制耐甲氧西林金黄色葡萄球菌（MRSA）。BBR@SP凝胶还抑制和破坏生物膜形成，并下调毒力因子的表达。我们发现BBR@SP凝胶可以通过促进血管生成、皮肤再生和抑制炎症反应来加速MRSA感染的糖尿病伤口愈合。我们的工作提出了一种创新的抗菌策略，通过协同化学-光动力杀灭作用消除耐药菌，同时缓解生物膜缺氧，并阻断QS，这可能为解决抗菌药物耐药性和对抗生物膜相关感染开辟新的前景。(c)2021爱思唯尔有限公司保留所有权利。
Interaction between bacterial species is through quorum sensing (QS), a process that regulates and coordinates genes in virulence activities and biofilm formation. QS contributes to the slowdown of tissue repair during the wound healing process by inducing chronic inflammation of the wound. Here, we developed a multifunctional QS inhibitor based on a bioactive hydrogel system to interrupt the QS of bacteria, relieve hypoxia, and destroy biofilms, and thus accelerate the healing of infected wounds in diabetic mice. We loaded berberine (BBR, a QS inhibitor, and antibacterial agent) into a natural living microalgae Spirulina platensis (SP) to form a bioactive hydrogel (BBR@SP gel) in combination with carboxymethyl chitosan/sodium alginate. Under laser irradiation, the BBR@SP gel could constantly release BBR and produce reactive oxygen species, resulting in a synergistic QS inhibition against methicillin-resistant Staphylococcus aureus (MRSA) combined chemo-photodynamic therapy. The BBR@SP gel also suppresses and destroys biofilm formation and down-regulates the expression of virulence factors. We found that the BBR@SP gel could accelerate MRSA-infected diabetic wound healing by promoting angiogenesis, skin regeneration, and suppressing the inflammatory response. Our work presents an innovative antimicrobial strategy that eliminates drug-resistant bacteria by synergistic chemo-photodynamic killing effect, relieving biofilm hypoxia, and blocking QS simultaneously, which may open up new prospects in solving antimicrobial drug resistance and combating biofilm-related infections.(c) 2021 Elsevier Ltd. All rights reserved.