Arachidonic acid enriched live prey induces albinism in Senegal sole (Solea senegalensis) larvae
Arachidonic acid enriched live prey induces albinism in Senegal sole (Solea senegalensis) larvae
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DOI：
10.1016/j.aquaculture.2004.11.035
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发表时间：
2005-03
期刊：
影响因子：
4.5
通讯作者：
M. Villalta;A. Estévez;M. Bransden
中科院分区：
文献类型：
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作者：
M. Villalta;A. Estévez;M. Bransden
我们采用剂量反应设计研究了膳食花生四烯酸（20:4n-6，ARA）对塞内加尔鳎幼虫生长、存活、色素沉着和脂肪酸组成的影响。从孵化后 3 到 37 天 (dph)，幼虫喂食活饵料（轮虫从 3 dph 到 9 dph，卤虫无节幼体从 -37 dph），这些食物已使用含有 3 个分级浓度（1.3、68 和 120.1 mg ARA g−1 干重）的 ARA 和恒定二十二碳六烯酸（22:6n-3， DHA）。使用商业浓缩产品（DHA-蛋白质 Selco，ARA 含量 7.8 mg g−1 干重）作为参考饮食。卤虫无节幼体中 ARA 的最终浓度范围为 0.2 至 17.5 mg g−1 脂质。生长和存活与测试的 ARA 饮食水平无关。然而，膳食 ARA 与导致白化病增加的色素沉着显着减少之间存在相关性。组织脂肪酸浓度反映了相应的膳食成分。所有检查组织中的二十碳五烯酸（20:5n-3，EPA）水平与膳食 ARA 呈负相关。相对于饮食，组织中二十二碳五烯酸（22:5n-3，DPA）的比例几乎增加了 100 倍，这可能表明由于饮食中 DHA 不足而导致 EPA 链延长。研究发现，色素沉着率与头部 ARA 含量以及膳食 ARA/EPA 比率之间存在负线性相关关系。
We examined the effect of dietary arachidonic acid (20:4n-6, ARA) on growth, survival, pigmentation and fatty acid composition of Senegal sole larvae using a dose–response design. From 3 to 37 days post-hatch (dph), larvae were fed live food (rotifers from 3 to 9 dph, Artemia nauplii from −37 dph) that had been enriched using one of three experimental emulsions containing 3 graduated concentrations (1.3, 68 and 120.1 mg ARA g−1dry weight) of ARA and constant docosahexaenoic acid (22:6n-3, DHA). A commercial enrichment product (DHA-Protein Selco, ARA content 7.8 mg g−1dry weight) was used as a reference diet. Final concentration of ARA in Artemia nauplii ranged from 0.2 to 17.5 mg g−1lipids. Growth and survival were independent of dietary levels of ARA tested. However, there was a correlation between dietary ARA and a significant reduction in pigmentation leading to increased albinism. Tissue fatty acid concentrations reflected the corresponding dietary composition. Eicosapentaenoic acid (20:5n-3, EPA) levels in all the tissues examined were inversely related to dietary ARA. There was almost a 100-fold increase in the proportion of docosapentaenoic acid (22:5n-3, DPA) in the tissues relative to the diet, which might indicate chain elongation from EPA as a result of inadequate dietary DHA. A negative, linear correlation was found between the pigmentation rate and the ARA content in the head, as well as with dietary ARA/EPA ratio.