Occurrence of Two Distinct Theileria Lineages in Sika Deer (Cervus nippon) of Iwate Prefecture, Japan
Occurrence of Two Distinct Theileria Lineages in Sika Deer (Cervus nippon) of Iwate Prefecture, Japan
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DOI：
10.1292/jvms.11-0094
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发表时间：
2011-10-01
影响因子：
1.2
通讯作者：
Itagaki, Tadashi
中科院分区：
文献类型：
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作者：
Ikawa, Kazuhito;Aoki, Mikiko;Itagaki, Tadashi
本研究用聚合酶链式反应检测了日本岩手县141头梅花鹿(Cervus Nippon)的肝脏或血液中的原生动物血液寄生虫。通过扩增18S rRNA基因V4高变区，在141份样品中有76份(53.9%)扩增出约500个碱基的扩增产物，这些扩增产物被认为是泰勒氏菌的DNA。测定了25份标本的18S rRNA基因全序列(1701-bp)，并将其分为8个基因类型，它们在系统发育上分为两个不同的谱系，显示出单系关系。从岩手县梅花鹿中检出泰勒氏杆菌两个谱系的比例不同，分别为12%和88%。
In the present study, we tried to detect protozoan blood parasites from the liver or blood of 141 Japanese sika deer (Cervus nippon) in Iwate Prefecture of Japan by polymerase chain reaction. Approximately 500-bp amplicons were obtained in 76 (53.9%) of 141 samples by amplification for V4 hyper-variable regions of the 18S rRNA gene, and the amplicons were considered to be DNA of Theileria species. The complete nucleotide sequences (1701-bp) of the 18S rRNA gene were determined in 25 samples and were divided into 8 genotypes that were phylogenetically separated into two distinct lineages showing a monophyletic relation. The two lineages of Theileria were detected in different rates (12 and 88%) from sika deer in Iwate Prefecture.