A reversibly antigen-responsive hydrogel
A reversibly antigen-responsive hydrogel
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DOI：
10.1038/21619
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发表时间：
1999-06-24
期刊：
影响因子：
64.8
通讯作者：
Uragami, T
中科院分区：
文献类型：
--
作者：
Miyata, T;Asami, N;Uragami, T
刺激响应性水凝胶响应于外部刺激（例如pH、温度和溶剂组成）而经历体积的突然变化，在生物医学和“智能”材料系统的创建中具有潜在的应用，例如作为用于药物递送、分离过程和蛋白质固定的介质。已报道水凝胶响应于pH（1-3）、温度（4- 1 - 3）、电场（14-16）和pH（17-22）。对于某些生物医学应用，具有其溶胀响应由特定蛋白质决定的材料将是非常有用的。在这里，我们报告这样一种材料，它在缓冲溶液中可逆地膨胀，以响应特定的抗原。通过将抗原和相应的抗体接枝到聚合物网络来制备水凝胶，使得两者之间的结合在网络中引入交联。由于这些非共价交联的破坏，游离抗原的竞争性结合引发凝胶体积的变化。此外，我们表明，水凝胶显示形状记忆行为，并且抗原浓度的逐步变化可以诱导蛋白质通过网络的脉动渗透。
Stimuli-responsive hydrogels that undergo abrupt changes in volume in response to external stimuli such as pH, temperature and solvent composition have potential applications in biomedicine and the creation of 'intelligent' materials systems, for example as media for drug delivery, separation processes and protein immobilization. Hydrogels have been reported that respond to pH(1-3), temperature(4-13), electric fields(14-16) and saccharides(17-22). For some biomedical applications it would be very useful to have a material whose swelling response was dictated by a specific protein. Here we report such a material, which swells reversibly in a buffer solution in response to a specific antigen. The hydrogel was prepared by grafting the antigen and corresponding antibody to the polymer network, so that binding between the two introduces crosslinks in the network. Competitive binding of the free antigen triggers a change in gel volume owing to breaking of these non-covalent crosslinks. In addition, we show that the hydrogel displays shape-memory behaviour, and that stepwise changes in antigen concentration can induce pulsatile permeation of a protein through the network.