Characterization of 50-kHz ultrasonic vocalizations in male and female rats
Characterization of 50-kHz ultrasonic vocalizations in male and female rats
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DOI：
10.1016/s0031-9384(03)00227-0
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发表时间：
2003-10-01
影响因子：
2.9
通讯作者：
Vakulenko, M
中科院分区：
文献类型：
--
作者：
McGinnis, MY;Vakulenko, M
雄性和雌性大鼠在生殖接触中都会发出超声波发声。虽然发情被用来诱导雄性发声，但反应的数量是不同的。我们报告了一种可靠的方法来评估发声使用暴露在刺激动物。将刺激大鼠置于金属丝屏障后5分钟，然后取出。然后记录发声5分钟。实验1验证了该方法，并将其用于后续实验。在实验2中，雄性大鼠被去势并进行发声恢复测试。在一组中，男性被允许自由交配；在另一组中，女性戴着防止射精的阴道口罩，但不能戴口罩。只有被允许射精的男性才能恢复发声。在实验3中，我们测量了性幼稚和性经验丰富的男性在暴露于去势(CAS)男性、睾酮(T)治疗男性、去卵巢(OVX)女性或接受雌激素+黄体酮(E+P)的OVX女性后的发声。男性在接触E+P女性后发声最多，无论他们是有性经验还是天真。然而，在与E+P女性接触后，当男性经历性行为时，发声的比率显著高于E+P女性。在实验4中，我们测量了女性在接触CAS男性、T处理男性、OVX女性或E+P女性后的发声。雌性在接触经T处理的雄性后发声最多。我们的结果表明：(1)性经验促进了雄性大鼠的发声，(2)暴露于激素接受性同种物质后发声最高，(3)超声信号是评估同种物质激素倾向的敏感指标。(C)2003 Elsevier Inc.保留所有权利。
Male and female rats emit ultrasonic vocalizations in reproductive encounters. While estrous bedding has been used to elicit vocalizations of males, the number of responses is variable. We report a reliable method to assess vocalizations using exposure to a stimulus animal. The stimulus rat is placed behind a wire barrier for 5 min, then removed. Vocalizations are then recorded for 5 min. Experiment 1 validated this method and it was used for subsequent experiments. In Experiment 2, male rats were castrated and tested for the restoration of vocalizations. In one group, males were allowed to copulate freely; in the other, females had vaginal masks to prevent ejaculation, but not mounting. Vocalizations were restored only in males allowed to ejaculate. In Experiment 3, we measured vocalizations in sexually naive and sexually experienced males following exposure to either castrated (CAS) males, testosterone (T)-treated males, ovariectomized (OVX) females, or OVX females receiving estrogen plus progesterone (E + P). Males vocalized most after exposure to E + P females, whether they were sexually experienced or naive. However, the rate of vocalizations was significantly higher after exposure to E + P females when the males were sexually experienced. In Experiment 4, we measured vocalizations in females following exposure to CAS males, T-treated males, OVX females, or E + P females. Females vocalized most after exposure to T-treated males. Our results show that (1) sexual experience facilitates vocalizations in male rats, (2) vocalizations are highest after exposure to hormonally receptive conspecifics, and (3) ultrasonic signaling is a sensitive index for assessing the hormonal disposition of conspecifics. (C) 2003 Elsevier Inc. All rights reserved.