Tensile behaviour of fibroblasts cultured in collagen gel.
Tensile behaviour of fibroblasts cultured in collagen gel.
复制标题
胶原凝胶中培养的成纤维细胞的拉伸行为。
DOI：
10.1016/0142-9612(96)87280-2
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发表时间：
1996
期刊：
影响因子：
14
通讯作者：
Hiroo Miyairi
中科院分区：
文献类型：
--
作者：
Kazuo Takakuda;Hiroo Miyairi
利用延时录像系统对应力场与细胞取向的关系进行了体外形态学研究。在不同的机械条件下，对细胞增殖的薄胶原凝胶膜标本进行了检查。记录了胶原凝胶中细胞拉伸、细胞取向、细胞迁移、细胞增殖和拉伸应力的产生。结果表明，成纤维细胞产生张力并沿张力方向改变其取向。对这种现象提出了一种假设的机制，即成纤维细胞产生张力并产生紧张的胶原纤维，然后细胞沿着紧张的纤维伸展并向这个方向增加张力。因此，这种机制作为一个正反馈回路，使细胞能够根据机械环境产生拉伸应力并沿着它们排列。
An in vitro morphological study on the relation of the stress field and the orientation of cells with the use of the time lapse video recording system was carried out. Specimens of thin collagen gel membrane, within which cells are proliferating, were examined under various mechanical conditions. Cell stretching, cell orientation, cell migration, cell proliferation and generation of tensile stress in collagen gel were recorded. It was demonstrated that fibroblasts generate tension and change their orientation along the tensile direction. A hypothetical mechanism for such phenomena is proposed, that is, fibroblasts generate tension and make tense collagen fibres, then cells stretch themselves along the tense fibres and increase tension in this direction. Thus, this mechanism works as a positive feedback loop which enables cells to make tensile stresses and align along them in accordance with the mechanical environment.