Synthesis and characterization of molecularly imprinted microspheres
Synthesis and characterization of molecularly imprinted microspheres
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DOI：
10.1021/ma000825t
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发表时间：
2000-10-31
期刊：
影响因子：
5.5
通讯作者：
Mosbach, K
中科院分区：
文献类型：
--
作者：
Ye, L;Weiss, R;Mosbach, K
开发了一种制备分子印迹微球的新方法。使用茶碱、咖啡因和 17β-雌二醇作为模板分子来证明普遍适用的方法。在模板分子存在下，从甲基丙烯酸和三羟甲基丙烷三甲基丙烯酸酯的稀溶液开始交联聚合，产生带有印迹结合位点的聚合物微球。放射性配体结合分析证实，当功能单体:交联剂的摩尔比控制在1:1至2.3:1之间时，保留了非常高的结合特异性。当用于聚合物制备的模板分子数量增加时，分子印迹微球的非特异性结合会减少。与之前报道的悬浮聚合和种子聚合相比，该方法效率高，普遍适用于各种模板分子，并且以良好的收率得到均匀的微球。
A novel method for the preparation of molecularly imprinted microspheres has been developed. Theophylline, caffeine, and 17 beta -estradiol are,used as template molecules to demonstrate the generally applicable methodology. In the presence of a template molecule, cross-linking polymerization starting from a dilute solution of methacrylic acid and trimethylolpropane trimethacrylate yields polymer microspheres bearing imprinted binding sites. Radioligand binding analysis confirms that very high binding specificity is retained when the molar ratio of functional monomer:cross-linker is controlled between 1:1 and 2.3:1. Nonspecific binding of molecularly imprinted microspheres decreases when an increasing amount of template molecule is used for polymer preparation. Compared with previously reported suspension polymerization and seed polymerization, the present method is highly efficient, is generally applicable to various template molecules, and gives uniform microspheres in good yield.