Bioluminescence imaging of dual gene expression at the single-cell level
Bioluminescence imaging of dual gene expression at the single-cell level
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DOI：
10.2144/000113419
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发表时间：
2010-06-01
期刊：
影响因子：
2.7
通讯作者：
Ohmiya, Yoshihiro
中科院分区：
文献类型：
--
作者：
Kwon, HyuckJoon;Enomoto, Toshiteru;Ohmiya, Yoshihiro
生物发光成像揭示了单个细胞中单个基因表达的长期动态。然而，迄今为止，用于同时成像多个基因表达模式的方法是未知的。在这里，我们构建了一个双光路光学发光成像系统，使用双色报告系统，可以同时跟踪两个基因的表达模式在一个单一的细胞数天。
Bioluminescence imaging reveals the long-term dynamics of individual gene expression in a single cell. However, methods for simultaneously imaging multiple gene expression patterns have been unknown to date. Here, we constructed a dual-path optical luminescence imaging system using a two-color reporter system and could simultaneously track two gene expression patterns for several days in a single cell.