Cellular properties of principal neurons in the rat entorhinal cortex. I. The lateral entorhinal cortex
Cellular properties of principal neurons in the rat entorhinal cortex. I. The lateral entorhinal cortex
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DOI：
10.1002/hipo.20997
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发表时间：
2012-06-01
期刊：
影响因子：
3.5
通讯作者：
Witter, Menno P.
中科院分区：
文献类型：
--
作者：
Canto, Cathrin B.;Witter, Menno P.
外侧内嗅皮层（LEC）提供了一个主要的皮质输入到海马结构，相当于内侧内嗅皮层（MEC）。为了理解LEC的功能贡献，需要在内在网络的背景下了解单个神经元的基本知识。本研究的目的是比较生后大鼠LEC不同层主要神经元的生理和形态学特性。使用在体外全细胞电流钳记录从多达四个事后形态学鉴定的神经元同时，我们建立了主神经元显示层特定的生理和形态学特性，类似于以前报道的成人。在L（艾耶尔）I，LII和LIII的主要神经元有大部分的树突和轴突侧支单独在浅层。LV主要包含锥体神经元，树突和轴突延伸遍及所有层。少数LV和LVI的所有主要神经元都是树突局限于深层而轴突分布于浅层和深层的神经元。在生理上，LII神经元的输入电阻和时间常数分别比LV神经元中观察到的低和短。54%的LII神经元在超极化电流注入的偏移时具有下垂电位、共振特性和反弹，而LIII和LVI神经元不具有这些。LV神经元表现出明显的尖峰频率适应和尖峰幅度的下降，在强去极化。尽管在LEC发达的层间通信，神经元的生物物理和形态学特性的层状差异表明，它们在体内的放电模式和功能不同，类似于已知的神经元在不同的MEC层。(c)2011 Wiley Periodicals，Inc.
The lateral entorhinal cortex (LEC) provides a major cortical input to the hippocampal formation, equaling that of the medial entorhinal cortex (MEC). To understand the functional contributions made by LEC, basic knowledge of individual neurons, in the context of the intrinsic network, is needed. The aim of this study is to compare physiological and morphological properties of principal neurons in different LEC layers in postnatal rats. Using in vitro whole cell current-clamp recordings from up to four post hoc morphologically identified neurons simultaneously, we established that principal neurons show layer specific physiological and morphological properties, similar to those reported previously in adults. Principal neurons in L(ayer) I, LII, and LIII have the majority of their dendrites and axonal collaterals alone in superficial layers. LV contains mainly pyramidal neurons with dendrites and axons extending throughout all layers. A minority of LV and all principal neurons in LVI are neurons with dendrites confined to deep layers and axons in superficial and deep layers. Physiologically, input resistances and time constants of LII neurons are lower and shorter, respectively, than those observed in LV neurons. Fifty-four percent of LII neurons have sag potentials, resonance properties, and rebounds at the offset of hyperpolarizing current injection, whereas LIII and LVI neurons do not have any of these. LV neurons show prominent spike-frequency adaptation and a decrease in spike amplitudes in response to strong depolarization. Despite the well-developed interlaminar communication in LEC, the laminar differences in the biophysical and morphological properties of neurons suggest that their in vivo firing patterns and functions differ, similar to what is known for neurons in different MEC layers. (c) 2011 Wiley Periodicals, Inc.