Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.
Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.
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DOI：
10.1038/srep13702
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发表时间：
2015-09-08
影响因子：
4.6
通讯作者：
Cizkova D
中科院分区：
文献类型：
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作者：
Grulova I;Slovinska L;Blaško J;Devaux S;Wisztorski M;Salzet M;Fournier I;Kryukov O;Cohen S;Cizkova D
脊髓损伤（SCI）涉及神经细胞损失，从而导致功能性运动和感觉障碍。在这项研究中，我们提出了一种以海藻酸盐为基础的富含/不含营养生长因子（GFs）的神经桥，可以作为脊髓修复的治疗方法。从注射海藻酸盐生物材料中释放的表皮生长因子（EGF）和碱性成纤维细胞生长因子（bFGF）等关键生长因子的生物利用度显著增强脊髓组织的保留，并增加存活神经元（胆碱乙酰转移酶阳性运动神经元）和感觉纤维的数量。此外，我们还记录了皮质脊髓束轴突的增强生长和中央病变处血管的存在。脊髓损伤后第3、7和10天进行的组织蛋白质组学分析显示，在中心病变部位以上的节段存在抗炎因子，而在中心病变部位以下的节段，神经突生长因子、炎症因子和炎性蛋白家族的硫酸软骨素蛋白多糖过表达。总的来说，根据我们的数据，我们证实，与未接受生物材料治疗的SCI动物相比，接受带有亲和结合生长因子（ALG +GFs）的海藻酸盐支架的SCI组的功能恢复明显改善。
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate -based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG +GFs), compared to SCI animals without biomaterial treatment.