Resolution of chlamydial genital infection with antigen-specific T-lymphocyte lines.
Resolution of chlamydial genital infection with antigen-specific T-lymphocyte lines.
复制标题
用抗原特异性 T 淋巴细胞系解决衣原体生殖器感染。
DOI：
10.1128/iai.59.3.925-931.1991
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发表时间：
1991
影响因子：
3.1
通讯作者：
Rank,RG
中科院分区：
文献类型：
--
作者：
Ramsey,KH;Rank,RG
为了确定涉及衣原体生殖器感染的小鼠的分辨率的细胞介导的免疫机制，我们利用了一个建立的小鼠模型，其中已经证明，感染的分辨率发生独立的抗体反应。脾T淋巴细胞从先前用小鼠肺炎因子（MoPn）（沙眼衣原体生物型）的活的基本体免疫的小鼠获得。在抗原呈递细胞和重组白细胞介素-2（rIL-2）的存在下，通过用UV光灭活的MoPn频繁再刺激来维持和扩增抗原反应性T淋巴细胞。流式细胞术表明，该细胞系是至少92%的泛特异性T细胞标志物Thy1.2阳性。在存在同源抗原呈递细胞加MoPn抗原的情况下和在不存在外源性IL-2的情况下刺激细胞诱导细胞在培养上清液中产生IL-2活性。继过继转移，这种T淋巴细胞系是有效的诱导决议正在进行的MoPn生殖器感染的先天性无胸腺裸鼠，否则保持慢性未解决的感染。在培养较长时间后，该细胞系在解决感染方面效率较低。另一种T淋巴细胞系来源于接受第一线治疗并已解决感染的无胸腺小鼠的脾脏。这些T细胞也能够诱导感染消退。最后，用特异性抗体和补体处理该细胞系以删除CD 4+或CD 8 + T淋巴细胞，试图在转移到感染的无胸腺小鼠中之前富集T细胞亚群。抗CD 4处理的细胞系基本上耗尽了CD 4细胞，而抗CD 8处理的细胞系仅部分富集了CD 4细胞，仍存在大比例的CD 8细胞。接受治疗的T细胞系或亲本细胞系的裸鼠能够解决感染，尽管具有增加数量的CD 4细胞的细胞系比亲本细胞系或CD 8细胞系更有效。
To determine cell-mediated immune mechanisms involved in the resolution of chlamydial genital infection of mice, we utilized an established murine model in which it has been demonstrated that resolution of infection occurs independently of the antibody response. Splenic T lymphocytes were obtained from mice that had previously been immunized with viable elementary bodies of the mouse pneumonitis agent (MoPn), a Chlamydia trachomatis biovar. Antigen-reactive T lymphocytes were maintained and expanded in vitro by frequent restimulation with UV light-inactivated MoPn in the presence of antigen-presenting cells and recombinant interleukin-2 (rIL-2). Flow cytometry indicated that this cell line was at least 92% positive for the pan-specific T-cell marker Thy1.2. Stimulation of the cells in the presence of syngeneic antigen-presenting cells plus MoPn antigen and in the absence of exogenous IL-2 induced the cells to produce IL-2 activity in culture supernatants. Following adoptive transfer, this T-lymphocyte line was effective in inducing resolution of an ongoing MoPn genital infection in congenitally athymic nude mice which otherwise maintain chronic unresolved infections. The line was less efficient in resolving the infection after longer periods in culture. An additional T-lymphocyte line was derived from the spleens of athymic mice that had received the first line and had resolved the infection. These T cells were also capable of inducing resolution of the infection. Lastly, this cell line was treated with specific antibody and complement to delete either CD4+ or CD8+ T lymphocytes in an attempt to enrich for T-cell subpopulations prior to transfer into infected athymic mice. The anti-CD4-treated line was essentially depleted of CD4 cells, while the anti-CD8-treated line was only partially enriched for CD4 cells, with a large proportion of CD8 cells still present. Nude mice that received either of the treated T-cell lines or the parental cell line were capable of resolving the infection, although the line with increased numbers of CD4 cells was more efficient than either the parental line or the CD8 line.