Toxicity and LD50 levels of the red tide dinoflagellate Heterocapsa circularisquama on juvenile pearl oysters
Toxicity and LD50 levels of the red tide dinoflagellate Heterocapsa circularisquama on juvenile pearl oysters
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赤潮甲藻 Heterocapsacircularisquama 对幼年珍珠贝的毒性和 LD50 水平
DOI：
10.1016/s0044-8486(96)01307-5
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发表时间：
1996
期刊：
影响因子：
4.5
通讯作者：
T. Honjo
中科院分区：
文献类型：
--
作者：
Kiyohito Nagai;Y. Matsuyama;T. Uchida;M. Yamaguchi;Misa Ishimura;A. Nishimura;S. Akamatsu;T. Honjo
对1992年发生在日本阿果湾的赤潮甲藻Heterocapsa circuliquama引起的合浦珠母贝（Pinctada fucata）大量死亡进行了实验室研究。用不同浓度的H.环弯藻、赤潮异弯藻和不育对照。H.当珠母贝幼贝的壳膜迅速收缩并关闭壳瓣时，然后，他们收缩他们的鳃，经历不规则的心跳模式，直到心脏永久停止。幼鱼的死亡率与H.圆鳞实验开始后24 h的LD 50约为20 000 cells ml-1，48 h后为10000 cells ml-1。然而，在H. akashiwo在100 000 cells ml-1或H.环斑牡蛎养殖72 h内幼贝未死亡。1992年阿戈湾赤潮发生时，赤潮藻细胞密度最大值为1.24 × 10 - 4，最大值为1.24 × 10 - 4.循环细胞达到80000个细胞ml-1以上。因此，我们认为这次赤潮中养殖珠母贝的大量死亡是由H.环状鳞状细胞
Laboratory experiments were conducted to clarify the mechanism of the mass mortality of pearl oysters (Pinctada fucata) associated with the red tide dinoflagellate Heterocapsa circularisquama such as occurred in Ago Bay, Japan, in 1992. Two-month-old pearl oysters were treated with various densities of H. circularisquama, Heterosigma akashiwo and a sterile control. Immediately after the exposure to H. circularisquama cells, juvenile pearl oysters rapidly contracted their mantles and closed their shell valves. Then, they contracted their gills and experienced irregular heartbeat patterns until the heart stopped permanently. The mortality of the juveniles was closely correlated with the cell density of H. circularisquama. The LD50was approximately 20 000 cells ml−1after 24 h and 10000 cells ml−1after 48 h from the start of the experiments. However, in the presence of either H. akashiwo at 100 000 cells ml−1or supernatant of H. circularisquama culture, juvenile pearl oysters did not die within 72 h. During the red tide in Ago Bay, 1992, the maximum cell density of H. circularisquama reached over 80 000 cells ml−1. Therefore, we consider that the mass mortality of cultured pearl oysters during this red tide was caused by the direct action of H. circularisquama cells.
影响因子：
1.5
作者：
Horiguchi, Takeo
通讯作者：
Horiguchi, Takeo
影响因子：
0.4
作者：
幸彦 松山;清仁 永井;忠久 水口;正巳 藤原;美佐 石村;峰生 山口;卓志 内田;凡夫 本城
通讯作者：
幸彦 松山;清仁 永井;忠久 水口;正巳 藤原;美佐 石村;峰生 山口;卓志 内田;凡夫 本城