Clones of the malaria parasite Plasmodium falciparum obtained by microscopic selection: their characterization with regard to knobs, chloroquine sensitivity, and formation of gametocytes.
Clones of the malaria parasite Plasmodium falciparum obtained by microscopic selection: their characterization with regard to knobs, chloroquine sensitivity, and formation of gametocytes.
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通过显微镜选择获得的疟疾寄生虫恶性疟原虫的克隆：其关于旋钮、氯喹敏感性和配子体形成的特征。
DOI：
10.1073/pnas.78.10.6527
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发表时间：
1981
影响因子：
11.1
通讯作者：
E. Gubert
中科院分区：
文献类型：
--
作者：
W. Trager;M. Tershakovec;L. Lyandvert;H. Stanley;N. Lanners;E. Gubert
使用恶性疟原虫（FCR-3/冈比亚）的培养系来选择含有单一寄生虫的细胞并将其置于培养物中。该方法依赖于油浸相差显微镜检查稀释细胞悬浮液的微小液滴。将发现含有单一寄生虫的液滴保持在适当的培养条件下，直到存在可检测数量的寄生虫（通常到第21天）。在长大的九个克隆体中，七个是无节的，两个是多节的。这些克隆对氯喹的敏感性略有不同。在一组体外条件下，它们的50%抑制点范围为0.02至0.06微克碱/毫升，而高敏感系FCR-8/西非只有0.003微克。所有三个测试克隆在适当的体外条件下形成配子母细胞。
A culture line of Plasmodium falciparum (FCR-3/Gambia) was used to select and place in culture cells containing a single parasite. The method depends on examination of minute droplets of dilute cell suspensions with oil immersion phase-contrast microscopy. Droplets found to contain a single parasite were maintained under appropriate culture conditions until detectable numbers of parasites were present (generally by day 21). Of nine clones that grew up, seven were knobless and two were knobby. The clones differed somewhat in chloroquine sensitivity. Their 50% inhibition point under one set of conditions in vitro ranged from 0.02 to 0.06 micrograms of base per ml, compared with only 0.003 micrograms for the highly sensitive line FCR-8/West Africa. All three tested clones formed gametocytes under appropriate in vitro conditions.