Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks
Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks
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DOI：
10.1007/s10858-011-9489-1
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发表时间：
2011-04-01
影响因子：
2.7
通讯作者：
Turano, Paola
中科院分区：
文献类型：
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作者：
Bernini, Patrizia;Bertini, Ivano;Turano, Paola
尿液、血清和血浆的 H-1 NMR 代谢分析已用于监测预分析步骤对样品质量和稳定性的影响，以便提出生物库沉积的标准操作程序 (SOP)。我们分析了血清和血浆样品的质量，作为血液采集和处理之间经过的时间（t = 0-4 小时）以及从处理到冷冻的时间（最多 24 小时）的函数。监测不同储存温度下尿液代谢特征随时间（最多 24 小时）的稳定性，作为不同分析前处理（如储存前离心、过滤和添加抑菌防腐剂叠氮化钠）的函数。在血液和尿液样本的化学和酶反应方面检测和讨论了曲线的明显变化，反映了许多代谢物浓度的变化。适当的血液衍生物收集和尿液保存/储存程序应运而生，以尽可能保持新鲜样本的原始代谢特征，并建议作为生物样本库的标准操作程序。
H-1 NMR metabolic profiling of urine, serum and plasma has been used to monitor the impact of the pre-analytical steps on the sample quality and stability in order to propose standard operating procedures (SOPs) for deposition in biobanks. We analyzed the quality of serum and plasma samples as a function of the elapsed time (t = 0-4 h) between blood collection and processing and of the time from processing to freezing (up to 24 h). The stability of the urine metabolic profile over time (up to 24 h) at various storage temperatures was monitored as a function of the different pre-analytical treatments like pre-storage centrifugation, filtration, and addition of the bacteriostatic preservative sodium azide. Appreciable changes in the profiles, reflecting changes in the concentration of a number of metabolites, were detected and discussed in terms of chemical and enzymatic reactions for both blood and urine samples. Appropriate procedures for blood derivatives collection and urine preservation/storage that allow maintaining as much as possible the original metabolic profile of the fresh samples emerge, and are proposed as SOPs for biobanking.