Genotype effect on regulation of behaviour by vitellogenin supports reproductive origin of honeybee foraging bias
Genotype effect on regulation of behaviour by vitellogenin supports reproductive origin of honeybee foraging bias
复制标题
DOI：
10.1016/j.anbehav.2010.02.009
复制
发表时间：
2010-05-01
期刊：
影响因子：
2.5
通讯作者：
Amdam, Gro V.
中科院分区：
文献类型：
--
作者：
Ihle, Kate E.;Page, Robert E., Jr.;Amdam, Gro V.
在蜂群中，食物的收集是由一群大多数不育的雌性工蜂完成的。在最初的筑巢阶段之后，工蜂开始觅食花蜜和花粉，但往往倾向于将它们的收集偏向于其中一个。虽然蜜蜂的觅食选择受到卵黄蛋白原（一种蛋黄前体蛋白）的影响，但工蜂通常不产卵。觅食生殖地面计划假说（RGPH）提出了一个进化的路径，其中收集花蜜或花粉觅食旅行的行为偏见的影响，在生殖生理变化，如激素水平和卵黄蛋白原（VG）基因表达。最近，卵黄蛋白原和觅食行为之间的联系受到Oldroyd和Beekman（2008）的挑战，他们从他们的研究中得出结论，卵巢，特别是卵黄蛋白原，在蜜蜂的觅食行为中没有发挥作用。我们解决他们的挑战，直接操纵Vg表达的RNA干扰（RNAi）介导的基因敲低在两个蜜蜂基因型不同的觅食行为和生殖生理。我们发现，vg影响的食物装载决定的工人只有在基因型中，觅食开始的时间（年龄）也敏感的卵黄蛋白原水平。在第二个基因型，改变卵黄蛋白原水平并不影响觅食的开始或偏见。卵黄蛋白原对工蚁觅食开始时年龄的影响可以用双抑制假说（DHR）来解释，该假说描述了卵黄蛋白原和保幼激素（JH）之间的相互抑制关系，JH是一种影响许多昆虫发育、生殖和行为的内分泌因子。这些结果支持RGPH，并展示了它如何与蜜蜂行为控制的既定机制相交。(C)2010年，动物行为研究协会。由爱思唯尔有限公司出版。保留所有权利。
In honeybee colonies, food collection is performed by a group of mostly sterile females called workers. After an initial nest phase, workers begin foraging for nectar and pollen, but tend to bias their collection towards one or the other. Although foraging choices of honeybees are influenced by vitellogenin, an egg-yolk precursor protein, workers typically do not lay eggs. The forager reproductive ground plan hypothesis (RGPH) proposes an evolutionary path in which the behavioural bias towards collecting nectar or pollen on foraging trips is influenced by variation in reproductive physiology, such as hormone levels and vitellogenin (vg) gene expression. Recently, the connections between vitellogenin and foraging behaviour were challenged by Oldroyd & Beekman (2008), who concluded from their study that the ovary, and especially vitellogenin, played no role in foraging behaviour of bees. We address their challenge directly by manipulating vg expression by RNA interference (RNAi) mediated gene knockdown in two honeybee genotypes with different foraging behaviour and reproductive physiology. We found that vg affected the food-loading decisions of the workers only in the genotype in which the timing of foraging onset (by age) was also sensitive to vitellogenin levels. In the second genotype, changing vitellogenin levels did not affect foraging onset or bias. The effect of vitellogenin on workers' age at foraging onset is explained by the well-supported double repressor hypothesis (DHR), which describes a mutually inhibitory relationship between vitellogenin and juvenile hormone (JH), an endocrine factor that influences development, reproduction and behaviour in many insects. These results support the RGPH and demonstrate how it intersects with an established mechanism of honeybee behavioural control. (C) 2010 The Association for the Study of Animal Behaviour. Published by Elsevier Ltd. All rights reserved.