Hydrogen sulfide delays leaf yellowing of stored water spinach (Ipomoea aquatica) during dark-induced senescence by delaying chlorophyll breakdown, maintaining energy status and increasing antioxidative capacity
Hydrogen sulfide delays leaf yellowing of stored water spinach (Ipomoea aquatica) during dark-induced senescence by delaying chlorophyll breakdown, maintaining energy status and increasing antioxidative capacity
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DOI：
10.1016/j.postharvbio.2015.05.003
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发表时间：
2015-10-01
影响因子：
7
通讯作者：
Shen, Wenbiao
中科院分区：
文献类型：
--
作者：
Hu, Huali;Liu, Dan;Shen, Wenbiao
在12-14 ℃的黑暗诱导衰老过程中，内源硫化氢（H2S）代谢的变化，叶绿素降解，能量状态，和抗氧化能力的空心菜熏蒸与或没有外源H2S供体硫氢化钠（NaHS）进行了评估。与对照相比，NaHS处理导致内源H2S和叶绿素含量增加，同时H2S合成酶活性增加，叶绿素降解酶活性降低。电子显微镜证实，叶绿体的崩溃减少NaHS。H2S不仅维持了能量状态，而且降低了呼吸速率，提高了抗氧化能力。由于H2S合成抑制剂DL-炔丙基葡萄糖的使用抑制了H2S的产生，加剧了I.水生痛，这种反应可以通过外源性应用NaHS来挽救。然而，亚牛磺酸（H2S的清除剂）处理与对照组之间没有明显差异。这些结果表明，NaHS熏蒸诱导的内源H2S可能通过改善能量状况，提高抗氧化能力，抑制呼吸速率，从而延缓了紫茎泽兰的衰老。水生植物(C)2015爱思唯尔B. V.保留所有权利。
During dark-induced senescence at 12-14 degrees C, changes of endogenous hydrogen sulfide (H2S) metabolism, chlorophyll degradation, energy status, and antioxidant capacity of Ipomoea aquatica fumigated with or without exogenously applied H2S donor sodium hydrosulfide (NaHS) were assessed. Compared to the control, NaHS treatment brought about higher contents of endogenous H2S and chlorophyll, both of which were matched with increased activities of H2S synthetic enzymes and decreased activities of chlorophyll-degrading enzyme. Electron microscopy confirmed that the breakdown of chloroplasts was reduced by NaHS. H2S not only sustained the energy status, but also decreased the respiration rate and increased antioxidant capacity. The inhibition of H2S production caused by the application of its synthetic inhibitor (DL-propargylglucine) aggravated the yellowing of I. aquatica, and this response could be rescued by exogenously applied NaHS. Nevertheless, no obvious differences were observed between treatment of hypotaurine (a scavenger of H2S) and control. These results suggest that NaHS fumigation-induced endogenous H2S might improve the energy status, leading to enhancement of antioxidant capacity and inhibition of the respiration rate, and consequently, reducing the senescence of I. aquatica. (C) 2015 Elsevier B.V. All rights reserved.