Organotypic Hippocampal Slice Cultures as a Model of Limbic Epileptogenesis
Organotypic Hippocampal Slice Cultures as a Model of Limbic Epileptogenesis
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DOI：
10.1007/978-1-60327-263-6_11
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发表时间：
2009-01-01
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影响因子：
--
通讯作者：
Bausch, Suzanne B.
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文献类型：
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作者：
Bausch, Suzanne B.
器官型海马切片培养经历创伤，传入神经通路的细胞丢失或横断，神经回路重排，类似于可能导致获得性颞叶癫痫的事件。器官型海马切片培养可在体外维持数月，并表现出潜伏期，随后出现涉及齿状颗粒细胞的电图癫痫发作，这是人类和体内动物模型中癫痫发生和获得性癫痫的标志。与体内模型相比，器官型海马切片培养的优势在于，切片培养具有相对较短的潜伏期，可以用已知浓度的试剂快速简便地治疗，而不会产生不必要的全身副作用。它们也更适合于延时研究，并且需要更少的动物进行药物筛选和浓度反应分析。因此，体外器官型海马切片培养模型是一种有吸引力的替代体内模型，可以开始阐明突触重排和癫痫发生的分子和细胞机制。
Organotypic hippocampal Slice Cultures experience trauma, deafferentation due to cell loss or transection of afferent pathways, and neuronal circuitry rearrangements much like the events that can lead to acquired temporal lobe epilepsy. Organotypic hippocampal slice cultures can be maintained for months in vitro and exhibit a latent period followed by onset of electrographic seizures involving the dentate granule cells, which is a hallmark of epileptogenesis and acquired epilepsy in humans and in vivo animal models. The advantages of organotypic hippocampal slice cultures over in vivo models arc that slice cultures exhibit a relatively short latent period and can be treated quickly and easily with a known concentration of reagent without unwanted systemic side effects. They are also more amenable to time-lapse studies and require fewer animals for drug screening and concentration-response analyses. Thus, the in vitro organotypic hippocampal slice culture model is an attractive alternative to in vivo models to begin to elucidate the molecular and cellular mechanisms underlying synaptic rearrangements and epileptogenesis.