Significance of podoplanin expression in keratocystic odontogenic tumor
Significance of podoplanin expression in keratocystic odontogenic tumor
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DOI：
10.1111/j.1600-0714.2009.00851.x
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发表时间：
2010-01-01
影响因子：
3.3
通讯作者：
Kusama, Kaoru
中科院分区：
文献类型：
--
作者：
Okamoto, Eri;Kikuchi, Kentaro;Kusama, Kaoru
背景：牙源性角化囊性肿瘤（KCOT）最重要的临床特征是其局部破坏性行为的潜力、复发的倾向以及其起源于牙源性上皮。 KCOT 的临床特征与成釉细胞瘤 (AM) 相似。在组织学上，KCOT 与角化颌囊肿（正角化牙源性囊肿；OOC）不同。然而，目前基于科学的临床参数无法预测 KCOT 患者潜在的肿瘤行为、侵袭性和局部侵袭性。我们已经证明，平足蛋白（一种淋巴管内皮标记物）在 AM 中高表达。本研究的目的是确定 podoplanin 在将牙源性角化囊肿 (OKC) 从囊肿重新分类为肿瘤状态时的有用性。方法：使用抗 podoplanin 抗体对 57 个 OKC（46 个 KCOT 和 11 个 OOC）和 15 个含牙囊肿 (DC) 的石蜡包埋组织标本进行免疫组织化学检查。结果：免疫组织化学在 KCOT 的基质结缔组织中，大部分基底层和基底上层的细胞膜和细胞质、出芽基底细胞增殖区域、上皮巢和子囊肿的外周细胞中检测到了足足蛋白的反应性。在OOC和DC中，只有与炎症相关的病例Podoplanin呈阳性。结论：与OOC相比，Podoplanin在KCOTs中表达较强。 KCOT 中足足蛋白的染色模式可能与其肿瘤性质有关，并表明该蛋白在肿瘤侵袭性中的作用。
Background:The most important clinical features of the keratocystic odontogenic tumor (KCOT) are its potential for locally destructive behavior, a tendency to recur, and its origin in the odontogenic epithelium. The clinical features of KCOT are similar to those of ameloblastoma (AM). Histologically, KCOT is distinguished from jaw cyst with keratinization (orthokeratinized odontogenic cyst; OOC). However, current scientifically based clinical parameters cannot predict any potential for neoplastic behavior, or aggressive and localized invasiveness, in patients with KCOT. We have shown that podoplanin, a lymphatic endothelial marker, is highly expressed in AM. The purpose of this study was to determine the usefulness of podoplanin for reclassification of the odontogenic keratocyst (OKC) from cyst to tumor status.Methods:Paraffin-embedded tissue specimens of 57 OKCs (46 KCOTs and 11 OOCs) and 15 dentigerous cysts (DCs) were immunohistochemically examined using antibody against podoplanin.Results:Immunohistochemical reactivity for podoplanin was detected in the cell membrane and cytoplasm of most of the basal and suprabasal layer, areas of budding basal cell proliferation, epithelial nests and peripheral cells of daughter cysts in the stromal connective tissue in KCOTs. In the case of OOC and DC, only cases associated with inflammation were positive for podoplanin.Conclusion:Podoplanin is strongly expressed in KCOTs in comparison with OOCs. The pattern of staining for podoplanin in KCOT could be related to its neoplastic nature, and suggests a role of the protein in tumor invasiveness.