Comamonas jiangduensis sp. nov., a biosurfactant-producing bacterium isolated from agricultural soil.
Comamonas jiangduensis sp. nov., a biosurfactant-producing bacterium isolated from agricultural soil.
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DOI：
10.1099/ijs.0.045716-0
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发表时间：
2013-06
影响因子：
2.8
通讯作者：
Li-na Sun;Jun Zhang;Qing Chen;Jian He;Qin-fen Li;Shun-Peng Li
中科院分区：
文献类型：
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作者：
Li-na Sun;Jun Zhang;Qing Chen;Jian He;Qin-fen Li;Shun-Peng Li
从农业土壤中分离出一种新型的生物表面活性剂生产菌株，命名为 YW1(T)。使用多相方法研究其分类地位。这些细胞是短杆状、革兰氏阴性、不形成孢子且能运动。基于16S rRNA基因序列的系统发育分析表明，菌株YW1(T)属于丛毛单胞菌属，与水生丛毛单胞菌LMG 2370(T)(98.5%)、克氏丛毛单胞菌LMG 3475(T)(97.7%)和土丛丛毛单胞菌LMG 1253(T)(97.7%)具有最高的序列相似性。此外，针对这三个菌株的DNA-DNA杂交实验给出的结果明显低于70％的DNA-DNA相似性，因此证实该新菌株不属于先前描述的丛毛单胞菌属物种。主要的呼吸醌是泛醌-8。主要脂肪酸（>5%）为C16:0（30.1%）、汇总特征3（C16:1ω6c和/或C16:1ω7c；25.4%）、汇总特征8（C18:1ω6c和/或C18:1ω7c；15.3%）、C17:0环（7.4%）和C14:0（5.8%）。主要极性脂质为二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、未知磷脂和未知脂质。根据系统发育分析、DNA-DNA杂交、全细胞脂肪酸组成以及生化特征，菌株YW1(T)与所有已有效公布名称的丛毛单胞菌属物种有明显区别，应被归类为丛毛单胞菌属新种的代表，其名称为江都丛毛单胞菌属新种。十一月被提议。模式菌株是YW1(T) (=CCTCC AB 2012033(T)=KACC 16697(T))。
A novel biosurfactant-producing strain, designated YW1(T), was isolated from agricultural soil. Its taxonomic position was investigated using a polyphasic approach. The cells were short rods, Gram-negative, non-sporulating and motile. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YW1(T) was a member of the genus Comamonas, and showed highest sequence similarities to Comamonas aquatica LMG 2370(T) (98.5%), Comamonas kerstersii LMG 3475(T) (97.7%) and Comamonas terrigena LMG 1253(T) (97.7%). Furthermore, DNA-DNA hybridization experiments against these three strains gave results that were clearly lower than 70% DNA-DNA similarity, and consequently confirmed that this new strain does not belong to a previously described species of the genus Comamonas. The major respiratory quinone was ubiquinone-8. The major fatty acids (>5%) were C16:0 (30.1%), summed feature 3 (C16:1ω6c and/or C16:1ω7c; 25.4%), summed feature 8 (C18:1ω6c and/or C18:1ω7c; 15.3%), C17:0 cyclo (7.4%) and C14:0 (5.8%). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unknown phospholipids and unknown lipids. Based on the phylogenetic analysis, DNA-DNA hybridization, whole-cell fatty acid composition as well as biochemical characteristics, strain YW1(T) was clearly distinguishable from all species of the genus Comamonas with validly published names and should be classified as a representative of a novel species of the genus Comamonas, for which the name Comamonas jiangduensis sp. nov. is proposed. The type strain is YW1(T) (=CCTCC AB 2012033(T)=KACC 16697(T)).