Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system.
Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system.
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小球藻病毒 PBCV-1 CviAII 限制和修饰系统的表征。
DOI：
10.1093/nar/20.20.5351
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发表时间：
1992
影响因子：
14.9
通讯作者：
VanEtten,JL
中科院分区：
文献类型：
--
作者：
Zhang,Y;Nelson,M;Nietfeldt,JW;Burbank,DE;VanEtten,JL
从感染PBCV-1病毒的NC64A细胞中分离到第二个DNA定点(限制性)内切酶(R.CviAII)及其同源腺嘌呤DNA甲基转移酶(M.CviAII)。R.CviAII是细菌限制性内切酶NlaIII的异合子，识别CATG序列，不切割CmATG序列。然而，与NlaIII不同，NlaIII在G之后切割，不切割CmATG或CATG序列，CviAII在C和A之间切割，不受CATG甲基化的影响。克隆了M.CviAII和R.CviAII基因，并测定了它们的DNA序列。这些基因是头尾相连的，使得M.CviAII甲基转移酶基因的TAA终止密码子与R.CviAII内切酶的ATG翻译起始点重叠。R.CviAII是第一个被克隆和测序的小球藻病毒位点特异性内切酶基因。
A second DNA site-specific (restriction) endonuclease (R.CviAII) and its cognate adenine DNA methyltransferase (M.CviAII) were isolated from virus PBCV-1 infectedChlorellastrain NC64A cells. R.CviAII, a heteroschizomer of the bacterial restriction endonucleaseNlaIII, recognizes the sequence CATG, and does not cleave CmATG sequences. However, unlikeNlaIII, which cleaves after the G and does not cleave either CmATG ormCATG sequences,CviAII cleaves between the C and A and is unaffected bymCATG methylation. The M.CviAII and R.CviAII genes were cloned and their DNA sequences were determined. These genes are tandemly arranged head-to-tail such that the TAA termination codon of the M.CviAII methyltransferase gene overlaps the ATG translational start site of R.CviAII endonuclease. R.CviAII is the first chlorella virus site-specific endonuclease gene to be cloned and sequenced.