2-CHLOROADENOSINE INHIBITS COMPLEMENT-INDUCED REACTIVE OXYGEN METABOLITE PRODUCTION AND RECOVERY OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES ATTACKED BY COMPLEMENT
2-CHLOROADENOSINE INHIBITS COMPLEMENT-INDUCED REACTIVE OXYGEN METABOLITE PRODUCTION AND RECOVERY OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES ATTACKED BY COMPLEMENT
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DOI：
10.1016/0006-291x(85)90240-2
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
CAMPBELL, AK
中科院分区：
文献类型：
--
作者：
ROBERTS, PA;MORGAN, BP;CAMPBELL, AK
鲁米诺增强的化学发光表明，2-氯腺苷抑制补体诱导的人多形核白细胞活性氧代谢物的产生[PMN]。这种抑制作用被8-苯基茶碱逆转。[125I]标记的抗C9单克隆抗体与人PMN结合，表明从细胞膜上去除膜攻击复合物，并且表明2-氯腺苷抑制这一过程。该化合物还增加了补体诱导的PMN细胞裂解的百分比。
Luminol-enhanced chemiluminescence demonstrated that 2-chloroadenosine inhibited complement-induced reactive O2 metabolite production in human polymorphonuclear leukocyte [PMN]. This inhibition was reversed by 8-phenyltheophylline. Binding of a [125I]-labeled monoclonal antibody against C9 to human PMN demonstrated removal of membrane attack complexes from the cell membranes, and in addition indicated inhibition of this processes by 2-chloroadenosine. This compound also increased the percentage of complement-induced cell lysis of PMN.