Engineering an improved acellular nerve graft via optimized chemical processing
Engineering an improved acellular nerve graft via optimized chemical processing
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DOI：
10.1089/ten.2004.10.1346
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发表时间：
2004-09-01
影响因子：
--
通讯作者：
Schmidt, CE
中科院分区：
文献类型：
--
作者：
Hudson, TW;Liu, SY;Schmidt, CE
我们研究的长期目标是设计一种用于临床神经修复的脱细胞神经移植物，并用作研究神经再生过程中神经-细胞外基质相互作用的模型系统。为了开发这种脱细胞神经移植物模型，我们（1）检查了去污剂对周围神经组织的影响，（2）利用这些知识创建了一种无细胞的神经移植物，细胞外基质保存良好。使用组织化学和Western分析，确定每种去污剂对细胞和细胞外组织组分的影响。用洗涤剂Triton X-200、磺基甜菜碱-16和磺基甜菜碱-10创建优化的方案。这项研究是迄今为止最全面的检查洗涤剂对周围神经组织形态和蛋白质组成的影响。还提出了一种改进的化学脱细胞方案，其比目前的主要方案更能保留天然神经的内部结构。
The long-term goal of our research is to engineer an acellular nerve graft for clinical nerve repair and for use as a model system with which to study nerve - extracellular matrix interactions during nerve regeneration. To develop this model acellular nerve graft we ( 1) examined the effects of detergents on peripheral nerve tissue, and ( 2) used that knowledge to create a nerve graft devoid of cells with a well-preserved extracellular matrix. Using histochemistry and Western analysis, the impact of each detergent on cellular and extracellular tissue components was determined. An optimized protocol was created with the detergents Triton X-200, sulfobetaine-16, and sulfobetaine-10. This study represents the most comprehensive examination to date of the effects of detergents on peripheral nerve tissue morphology and protein composition. Also presented is an improved chemical decellularization protocol that preserves the internal structure of native nerve more than the predominant current protocol.