ER81 and CaMKIV identify anatomically and phenotypically defined subsets of mouse olfactory bulb interneurons
ER81 and CaMKIV identify anatomically and phenotypically defined subsets of mouse olfactory bulb interneurons
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DOI：
10.1002/cne.21293
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发表时间：
2007-06-01
影响因子：
2.5
通讯作者：
Baker, Harriet
中科院分区：
文献类型：
--
作者：
Saino-Saito, Sachiko;Cave, John W.;Baker, Harriet
多巴胺（DA）在嗅球（OB）中表型分化的机制尚未完全阐明，并且是一些争议的主题。前往肾小球层的OB - DA中间神经元起源于心室下区（SVZ）和吻侧迁移流（RMS）。目前的研究调查了钙/钙调素依赖性蛋白激酶IV （CaMKIV）单独或与Ets转录因子ER81一起是否对祖细胞迁移过程中的表型测定是必要的。在包括OB在内的大部分脑区，CaMKIV和ER81呈倒数分布。在SVZ中，只有ER81可以被证明。在RMS中，一个祖先亚群含有ER81，但很少含有CaMKIV，如果有的话。在OB中，CaMKIV的表达仅限于深颗粒细胞，与ER81有有限的重叠。ER81在深颗粒细胞中表达较弱。强标记发生在二尖瓣和肾小球层，ER81标记了表达酪氨酸羟化酶（TH）或绿色荧光蛋白的多巴胺能肾小球周围细胞，后者报告基因受9 kb的5' TH启动子控制。气味剥夺导致TH免疫反应性显著下降5.2倍，但ER81的免疫反应性下降相对较小，为1.7倍。在CaMKIV敲除小鼠中，TH的表达以及脑和球茎的大小没有变化。这些数据表明，ER81可能是DA神经元分化所必需的，但不是充分的，CaMKIV不直接参与TH基因的调控。
The mechanisms underlying dopamine (DA) phenotypic differentiation in the olfactory bulb (OB) have not yet been fully elucidated and are the subject of some controversy. OB DA interneurons destined for the glomerular layer were shown to originate in the subventricular zone (SVZ) and in the rostral migratory stream (RMS). The current study investigated whether calcium/calmodulin-dependent protein kinase IV (CaMKIV) either alone or together with the Ets transcription factor ER81 was necessary for phenotypic determination during migration of progenitors. In most brain areas, including the OB, CaMKIV and ER81 displayed a reciprocal distribution. In the SVZ, only ER81 could be demonstrated. In the RMS, a subpopulation of progenitors contained ER81, but few, if any, contained CaMKIV. In OB, CaMKIV expression, restricted to deep granule cells, showed limited overlap with ER81. ER81 expression was weak in deep granule cells. Strong labeling occurred in the mitral and glomerular layers, where ER81 colabeled dopaminergic periglomerular cells that expressed either tyrosine hydroxylase (TH) or green fluorescent protein, the latter reporter gene under control of 9-kb of 5' TH promoter. Odor deprivation resulted in a significant 5.2-fold decline in TH immunoreactivity, but ER81 exhibited a relatively small 1.7-fold decline in immunoreactivity. TH expression as well as brain and bulb size were unchanged in CaMKIV knockout mice. These data suggest that ER81 may be required but is not sufficient for DA neuron differentiation and that CaMKIV is not directly involved in TH gene regulation.