The prospects of protein nanocrystallography
The prospects of protein nanocrystallography
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DOI：
10.1107/s0907444902016608
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发表时间：
2002-11-01
影响因子：
2.2
通讯作者：
Abrahams, JP
中科院分区：
文献类型：
--
作者：
Bodenstaff, ER;Hoedemaeker, FJ;Abrahams, JP
蛋白质晶体学实验方法的小型化有几个优点。首先，它减少了鉴定结晶条件所需的蛋白质量，使罕见的天然蛋白质和复合物的晶体学研究成为可能。其次，在非常小的体积中可以获得更高水平的过饱和，从而可以探索额外的结晶条件。第三，有迹象表明，在非常小的体积中生长的蛋白质晶体可能更好地有序。第四，小型化和自动化齐头并进，为更容易和更可重复的实验开辟了前景。讨论了纳米晶学的发展进展，并指出了存在的瓶颈。
The miniaturization of protein crystallography's experimental method has several advantages. Firstly, it reduces the amount of protein required for identifying crystallization conditions, allowing crystallographic studies of rare natural proteins and complexes. Secondly, higher levels of supersaturation can be obtained in very small volumes, allowing the exploration of additional crystallization conditions. Thirdly, there are indications that protein crystals grown in very small volumes may be better ordered. Fourthly, miniaturization and automation go hand in hand, opening the prospects of easier and more reproducible experimentation. Progress in the development of nanocrystallography is discussed and the remaining bottlenecks are highlighted.