Sodium butyrate-induced apoptosis and ultrastructural changes in MCF-7 breast cancer cells
Sodium butyrate-induced apoptosis and ultrastructural changes in MCF-7 breast cancer cells
复制标题
丁酸钠诱导MCF-7乳腺癌细胞凋亡和超微结构变化
DOI：
10.3109/01913123.2016.1170083
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发表时间：
2016-07-01
影响因子：
1
通讯作者：
Wei, Lei
中科院分区：
文献类型：
--
作者：
Wang, Ying;Hu, Peng-Chao;Wei, Lei
摘要本研究探讨丁酸钠（NaB）对MCF-7乳腺癌细胞的影响，并分析其作用机制。在这里，我们证明了一定浓度的NaB可以有效地诱导MCF-7细胞凋亡。采用细胞计数试剂盒-8 （CCK-8）法检测细胞活力和凋亡率。Western blotting检测Bcl-2表达水平的变化。我们用显微镜观察细胞形态的变化。免疫荧光显示部分凋亡核。电镜显示核仁厚，染色质边缘，线粒体减少，液泡明显。总之，我们的发现阐明了NaB改变MCF-7细胞超微结构的形态学机制。
ABSTRACT This study investigated the effects of sodium butyrate (NaB) on Michigan Cancer Foundation-7 (MCF-7) breast cancer cells and analyzed the relevant mechanism. Here, we demonstrated that a certain concentration of NaB effectively induced MCF-7 cell apoptosis. Cell counting kit-8 (CCK-8) assay was used to detect cell viability and the apoptosis rate. Western blotting was used to detect changes in the Bcl-2 expression level. We observed cell shape changes with microscopy. Immunofluorescence revealed some apoptotic nuclei. Electron microscopy revealed thick nucleoli, chromatin margination, reduced mitochondria, and dramatic vacuoles. Collectively, our findings elucidated the morphological mechanism by which NaB changed the ultrastructure of MCF-7 cells.