A complex imprinted polymer membrane combined with solid phase fluorescence detection for selective analysis of L-phenylalanine in water-abundant samples
A complex imprinted polymer membrane combined with solid phase fluorescence detection for selective analysis of L-phenylalanine in water-abundant samples
复制标题
复杂的印迹聚合物膜与固相荧光检测相结合，用于选择性分析富含水分的样品中的 L-苯丙氨酸
DOI：
10.1039/c3ay40745d
复制
发表时间：
2013-01-01
影响因子：
3.1
通讯作者：
Li, Gongke
中科院分区：
文献类型：
--
作者：
Huang, Jianxiang;Hu, Yuling;Li, Gongke
制备了一种选择性、水相容性、一次性的配合物印迹聚合物（CIP）膜，用于l -苯丙氨酸的固相荧光检测。l -苯丙氨酸配位铝（III）作为配合物模板。用固相荧光信号监测与邻苯二醛原位衍生后的l -苯丙氨酸。对影响CIP膜性能的几个参数进行了研究和优化。所制备的CIP膜对l -苯丙氨酸具有良好的荧光响应和选择性。CIP膜对l -苯丙氨酸的线性响应范围为5.0 ~ 100 μ mol L-1，检出限为0.90 μ mol L-1。所制备的CIP膜可用于饮料和人尿中l -苯丙氨酸的分析，其回收率为83.5 ~ 101.3%，rsd为7.2% ~ 9.5%。并与高效液相色谱法进行了比较。结果表明，该方法对富水体系中l -苯丙氨酸的选择性测定具有满意的灵敏度、准确度和精密度。
A selective, water-compatible and disposable complex imprinted polymer (CIP) membrane was prepared for L-phenylalanine detection combined with solid phase fluorescence detection. L-Phenylalanine coordinated with aluminum(III) acted as a complex template. The solid phase fluorescent signal was monitored to quantify L-phenylalanine after in situ derivatization with o-phthalaldehyde. Several parameters determining the performance of the CIP membrane were studied and optimized. The resulting CIP membrane exhibited good fluorescent response and selectivity towards L-phenylalanine in aqueous environments. The linear response of the CIP membrane to L-phenylalanine was in the range of 5.0-100 mu mol L-1 with a limit of detection of 0.90 mu mol L-1. The prepared CIP membrane was successfully applied in the analysis of L-phenylalanine in drinks and human urine with recoveries of 83.5-101.3% and RSDs ranging from 7.2% to 9.5%. The proposed method was compared with a high performance liquid chromatography method. The results showed that the developed method has satisfactory sensitivity, accuracy and precision for the selective determination of L-phenylalanine in water-abundant systems.