23Na NMR relaxation study of the effects of conformation and base composition on the interactions of counterions with double-helical DNA.
23Na NMR relaxation study of the effects of conformation and base composition on the interactions of counterions with double-helical DNA.
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23Na NMR 弛豫研究构象和碱基组成对反离子与双螺旋 DNA 相互作用的影响。
DOI：
10.1021/bi00314a009
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
RecordJr,MT
中科院分区：
文献类型：
--
作者：
Nordenskiöld,L;Chang,DK;Anderson,CF;RecordJr,MT
Lars Nordenskiold，** DK Chang，Charles F.安德森和M.小托马斯·雷德 *摘要：本文测定了~（23）Na在含不同螺旋双链脱氧核糖核酸的盐水溶液中的核磁共振弛豫速率（Tf 1和T_（1））。这些测量是在三种具有不同整体构象的合成聚核苷酸上进行的，即poly-（dA-dT）-poly（dA-dT）（交替B-DNA）、低盐下的聚（dG-dC）·聚（dG-dC）（B-DNA）和Br-聚（dG-dC）-Br-聚（dG-dC）（左手Z-DNA），以及碱基组成不同的四种天然DNA：产气荚膜梭菌（26%GC）、小牛胸腺（40%GC），大肠杆菌（50%GC）和溶壁微球菌（72%GC）。除poly（dA-dT）· poly（dA-dT）外，所有DNA在21 ℃和4.7T磁场下的~（23）Na NMR谱均为非洛伦兹谱。这些非洛仑兹光谱分析的基础上的两个状态
Lars Nordenskiold,** DK Chang, Charles F. Anderson, and M. Thomas Record, Jr.* abstract: NMR relaxation rates (Tfl and T {1) have been determined for 23Na in aqueous salt solutionscontaining various types of helical double-stranded deoxyribonucleic acids. These measurements were performed on three synthetic po-lynucleotides having different overall conformations, poly-(dA-dT)-poly (dA-dT)(alternating B-DNA), poly (dG-dC)· poly (dG-dC) at low salt (B-DNA), and Br-poly (dG-dC)-Br-poly (dG-dC)(left-handed Z-DNA), and on four types of natural DNA differing in base composition, Clostridium perfringens (26% GC), calf thymus (40% GC), Escherichia coli (50% GC), and Micrococcus lysodeikticus (72% GC). For all types ofDNA investigated, except poly (dA-dT)· poly (dA-dT), the 23Na NMR spectra measured at 21 C and an applied field of 4.7 T are non-Lorentzian. These non-Lorentzian spectra were analyzed on the basis of the two-state