JAXA-GCF project - high-quality protein crystals grown under microgravity environment for better understanding of protein structure
JAXA-GCF project - high-quality protein crystals grown under microgravity environment for better understanding of protein structure
复制标题
JAXA-GCF项目——微重力环境下生长的高质量蛋白质晶体，以更好地了解蛋白质结构
DOI：
10.1007/bf02870406
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Tetsuo Tanaka
中科院分区：
文献类型：
--
作者：
Masaru Sato;Hiroaki Tanaka;K. Inaka;Shinichi Shinozaki;A. Yamanaka;S. Takahashi;M. Yamanaka;E. Hirota;S. Sugiyama;Mitsuyasu Kato;Chie Saito;S. Sano;M. Motohara;Tai Nakamura;Tomoyuki Kobayashi;S. Yoshitomi;Tetsuo Tanaka
自2003年以来，日本宇宙航空研究开发机构(JAXA，前NASDA)一直在每半年实施一次项目(JAXA-GCF)，以利用俄罗斯的运输系统在微重力环境中获得高质量的蛋白质晶体。在该项目中，蛋白质样品主要由日本用户提供，日本宇宙航空研究开发机构为他们提供了微重力结晶实验的技术和文书支持。对于该项目，日本宇宙航空研究开发机构为微重力实验构建了方便用户的支持服务，并提供了定期和频繁的飞行机会。为了简化和改进技术问题，日本宇宙航空研究开发机构基于反扩散技术，设计了一种在空间和地面都有效的凝胶管法结晶装置。日本宇宙航空研究开发机构还提供了地面技术，以便利用一维模拟有效地初步优化结晶条件，并在X射线衍射实验之前收集和冷冻保护晶体。这些改进大大提高了取得有用成果的成功率。总之，日本宇宙航空研究开发机构开发了在微重力下生长高质量蛋白质晶体的技术，这种晶体可以衍射到原子分辨率，以便通过X射线衍射实验更好地了解蛋白质的三维结构。
Since 2003, Japan Aerospace Exploration Agency (JAXA, former NASDA) has been conducting a project on a semi-annual basis (JAXA-GCF) to obtain high-quality protein crystals in the microgravity environment using the Russian transportation system. For this project, protein samples were mostly provided by Japanese users for whom JAXA provided technical and clerical support for crystallization experiments in microgravity. For the project, JAXA has constructed a user-friendly support service for microgravity experiments and provided regular and frequent flight opportunities. To simplify and improve technological matters, JAXA devised a gel-tube method crystallization device, which is effective both in space and on ground, based on the counter-diffusion technique. JAXA also provided ground-based techniques for efficient preliminary optimization of crystallization conditions using a 1-dimensional simulation and for harvesting and cryoprotecting crystals before X-ray diffraction experiments. These improvements have significantly increased the success rate of obtaining useful results. In conclusion, JAXA has developed technologies for growing, in microgravity, high-quality protein crystals, which may diffract up to atomic resolution, for a better understanding of 3-dimensional protein structures through X-ray diffraction experiments.