Analysis of Relationship Between Microbial and Methanogenic Biomass in Methane Fermentation
Analysis of Relationship Between Microbial and Methanogenic Biomass in Methane Fermentation
复制标题
甲烷发酵中微生物与产甲烷生物质的关系分析
DOI：
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发表时间：
2009
影响因子：
3
通讯作者：
M. Kubo
中科院分区：
文献类型：
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作者：
K. Kubota;Yuya Ozaki;Y. Matsumiya;M. Kubo
为分析甲烷发酵过程中微生物生物量与甲烷产量的关系，采用环境DNA分析方法，对甲烷发酵过程中细菌和古菌的总生物量进行了分析。在以产甲烷污泥为种子进行甲烷发酵的情况下，产甲烷时总BA生物量可达1.5 × 108 ~ 3.6 × 108个/ml。另一方面，土壤悬浮液用作种子; 14天的培养不产生甲烷。而总BA生物量达到1.5 × 108个/ml以上。利用荧光显微镜（辅酶F420）对产甲烷菌生物量进行计数，分析甲烷发酵过程中产甲烷菌生物量及产甲烷菌占总BA的比例。在产甲烷阶段，产甲烷菌生物量达到1.3 × 108个/ml，占总BA的70%以上。改变种子中产甲烷菌的比例，可以使产甲烷相移动。然而，产甲烷菌和其他微生物在产甲烷阶段的关系几乎是相似的。
To analyze the relationship between biomass of microorganisms and methane production, the total biomass of bacteria and archaea (BA) during methane fermentation was analyzed by the environmental DNA analysis method. In the case of using methanogenic sludge as a seed which is generally used for methane fermentation, the total BA biomass reached to 1.5 × 108 to 3.6 × 108 cells/ml when methane was produced. On the other hand, soil suspension was used as a seed; methane was not produced for 14-day cultivation. However, the total BA biomass reached to above 1.5 × 108 cells/ml. The methanogen biomass was counted by using a fluorescence microscope (coenzyme F420), and the methanogen biomass and the ratio of methanogens in the total of BA were analyzed during methane fermentation. At the methane-producing phase, the methanogen biomass reached to 1.3 × 108 cells/ml, and the ratio of methanogens was above 70% of the total BA. When the ratio of methanogens in a seed was changed, the methane-producing phase was moved. However, the relationship between methanogens and other microorganisms at the methane-producing phase was almost similar.