Poly(ε-caprolactone) films as a potential substrate for tissue engineering an epidermal equivalent
Poly(ε-caprolactone) films as a potential substrate for tissue engineering an epidermal equivalent
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DOI：
10.1016/s0928-4931(02)00015-2
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发表时间：
2002-05-31
期刊：
影响因子：
--
通讯作者：
Hutmacher, DW
中科院分区：
文献类型：
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作者：
Khor, HL;Ng, KW;Hutmacher, DW
对人角质形成细胞（HK）在溶剂浇铸和双向拉伸聚己内酯（PCL）膜上的细胞培养进行了初步研究。将溶剂流延膜上的细胞附着和增殖与市售伤口敷料进行比较，同时使用光、共聚焦激光和扫描电子显微镜（CLM和SEM）评估双轴拉伸膜上的细胞附着、增殖和活力。将溶剂流延片材双轴拉伸以产生5-15 μ m厚的膜。双轴拉伸膜被证明是更好的表皮基板，由于更好的柔韧性和强度质量比比溶剂流延片材。获得的图像显示，细胞附着在聚（ε-己内酯）膜上并增殖，并且在整个培养期间保持高百分比的活力。角质形成细胞呈健康的鹅卵石状，并呈连续单层增殖。这些结果表明，聚（ε-己内酯）膜将支持人角质形成细胞的附着和增殖，并有可能作为一种基质材料，用于组织工程的表皮等效物。(C)2002 Elsevier Science B. V.保留所有权利。
A preliminary cell culture study of human keratinocytes (HK) on solvent-cast and biaxially stretched poly(e-caprolactone) (PCL) films was carried out. Cell attachment and proliferation on solvent-cast films was compared with commercially available wound dressings while cell attachment, proliferation and viability on biaxially stretched films were assessed using light, confocal laser and scanning electron microscopy (CLM and SEM). Solvent-cast sheets were biaxially stretched to produce 5-15-mum-thick films. Biaxially stretched films were shown to be better epidermal substrates due to the better flexibility and strength to mass ratio than solvent-cast sheets. Images obtained showed that the cells attached and proliferated on poly(epsilon-caprolactone) films, and maintained high percentage of viability throughout the culture period. Keratinocytes exhibited healthy cobblestone morphology and proliferated as continuous monolayers. These results indicated that poly(epsilon-caprolactone) films would support the attachment and proliferation of human keratinocytes and have the potential to be applied as a matrix material for tissue engineering an epidermal equivalent. (C) 2002 Elsevier Science B.V. All rights reserved.