In vivo treatment with GDF-9 stimulates primordial and primary follicle progression and theca cell marker CYP17 in ovaries of immature rats.
In vivo treatment with GDF-9 stimulates primordial and primary follicle progression and theca cell marker CYP17 in ovaries of immature rats.
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DOI：
10.1210/endo.141.10.7732
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发表时间：
2000-10
期刊：
影响因子：
4.8
通讯作者：
U. Vitt;E. Mcgee;M. Hayashi;A. Hsueh
中科院分区：
文献类型：
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作者：
U. Vitt;E. Mcgee;M. Hayashi;A. Hsueh
生长分化因子 (GDF)-9 是卵母细胞产生的转化生长因子-β 超家族的一种含有胱氨酸结的激素。在 GDF-9 缺失小鼠中，卵泡发育在初级阶段被抑制，GDF-9 体外治疗可增强窦前卵泡的生长。未成熟雌性大鼠用重组 GDF-9 治疗 7 或 10 天。 10 天时，GDF-9 治疗增加了卵巢重量，同时初级卵泡和小窦前卵泡的数量分别增加了 30% 和 60%。此外，原始卵泡的数量减少了29%，但腔前大卵泡的数量没有受到影响。相比之下，FSH 治疗使小窦前卵泡和大窦前卵泡的数量分别增加了 36% 和 177%，但不影响初级和原始卵泡的数量。免疫印迹分析显示，用 GDF-9 处理后，卵巢匀浆中的 CYP17（一种卵泡膜细胞标记物）有所增加，而 FSH 则没有。目前的结果表明，用 GDF-9 进行体内治疗可增强原始卵泡和初级卵泡向小窦前卵泡的进展。因此，除了广泛使用的 FSH（主要作用于更高级卵泡的发育）之外，GDF-9 治疗还可以提供另一种刺激早期卵泡发育的方法。
Growth differentiation factor (GDF)-9 is a cystine knot-containing hormone of the transforming growth factor-beta superfamily produced by the oocyte. In GDF-9 null mice, follicle development is arrested at the primary stage and GDF-9 treatment in vitro enhances preantral follicle growth. Immature female rats were treated with recombinant GDF-9 for 7 or 10 days. At 10 days, treatment with GDF-9 augmented ovarian weights, concomitant with an increase in the number of primary and small preantral follicles by 30 and 60%, respectively. Furthermore, the number of primordial follicles was decreased by 29%, but the number of large preantral follicles was not affected. In contrast, treatment with FSH increased the number of small and large preantral follicles by 36 and 177% but did not influence the number of primary and primordial follicles. Immunoblot analysis showed an increase of CYP17, a theca cell marker, in the ovarian homogenate after treatment with GDF-9 but not FSH. The present results indicate that in vivo treatment with GDF-9 enhances the progression of primordial and primary follicles into small preantral follicles. Thus, GDF-9 treatment could provide an alternative approach to stimulate early follicle development in addition to the widely used FSH that acts mainly on the development of more advanced follicles.