Comparative study between anion-exchange HPLC and homogeneous assay methods in regard to the accuracy of high- and low-density lipoprotein cholesterol measurement
Comparative study between anion-exchange HPLC and homogeneous assay methods in regard to the accuracy of high- and low-density lipoprotein cholesterol measurement
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DOI：
10.1016/j.clinbiochem.2007.07.008
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发表时间：
2007-11-01
影响因子：
2.8
通讯作者：
Tada, Norio
中科院分区：
文献类型：
--
作者：
Kurosawa, Hideo;Yoshida, Hiroshi;Tada, Norio
目的：最近建立了一种基于阴离子交换高效液相色谱法测定脂蛋白(高密度脂蛋白、低密度脂蛋白、低密度脂蛋白、极低密度脂蛋白和乳清蛋白)中胆固醇水平的简便方法。设计与方法：对包括3例胆汁淤积症患者的血清标本，分别用Cholestest-ldl和CholstestN-hdl(东京第一化学公司)均相法和高效液相色谱法(J Lipid Res 2003；44：1404-12)进行测定。结果：高密度脂蛋白均相法与高效液相色谱法测定高密度脂蛋白高度相关(r=0.976)。两个胆汁淤积症患者的样品不能用均相法测定，但用高效液相色谱法测定。低密度脂蛋白均一化法与高效液相色谱法测定低密度脂蛋白胆固醇有一定的相关性(r=0.823)。在此相关性分析中，观察到三个来自胆汁淤积症患者的异常样本，其血清胆固醇水平&17 mm o l/L。均一化验数据显示这些低密度脂蛋白的胆固醇水平为15.2~34.7 mmol/L，而高效液相色谱数据显示这些低密度脂蛋白的水平为3.6~8.2 mm ol/L，并且主要的脂蛋白组分是极低密度脂蛋白和低密度脂蛋白。低密度脂蛋白水平的差异(均一化验数据减去高效液相数据)与极低密度脂蛋白水平呈正相关。结论：在测定胆汁淤积症患者的样本时，均一化验可能会得出不准确的结果。相比之下，高效液相色谱法很可能能够准确地测量高密度脂蛋白和低密度脂蛋白的水平，而不涉及极低密度脂蛋白。(C)2007年加拿大临床化学家协会。爱思唯尔公司出版，版权所有。
Objectives: A convenient method based on anion-exchange HPLC was recently developed to determine cholesterol levels of lipoproteins (HDL, LDL, IDL, VLDL, and chylomicron). The present study was performed to compare this HPLC method to homogenous assay in regard to measurement accuracy of HDL and LDL cholesterol.Design and methods: Serum samples (n = 105), including three samples from cholestasis patients, were measured by homogenous assay with Cholestest-LDL and CholestestN-HDL (Daiichi Chemicals, Tokyo) and by HPLC as reported previously (J Lipid Res 2003; 44: 1404-12).Results: The homogenous assay for HDL cholesterol correlated strongly with the HPLC method for HDL cholesterol (r=0.976). Two samples from cholestasis patients could not be measured by homogenous assay but were measured by HPLC. The homogenous assay for LDL cholesterol correlated modestly with the HPLC method for LDL cholesterol (r=0.823). Three outlier samples, from cholestasis patients with serum cholesterol levels > 17 mmol/L, were observed in this correlation analysis. Homogenous assay data showed that these LDL cholesterol levels were 15.2-34.7 mmol/L. However, HPLC data showed that these LDL cholesterol levels were 3.6-8.2 mmol/L, and that the major lipoprotein fractions were VLDL and IDL. The difference in LDL cholesterol levels (homogenous assay data minus HPLC data) was positively correlated with VLDL cholesterol levels.Conclusions: When measuring samples from cholestasis patients, homogenous assay may give inaccurate results. In contrast, the HPLC method is likely to be capable of accurately measuring HDL and LDL cholesterol levels without the involving VLDL. (C) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.