Optimization of exopolysaccharide welan gum production by Alcaligenes sp CGMCC2428 with Tween-40 using response surface methodology
Optimization of exopolysaccharide welan gum production by Alcaligenes sp CGMCC2428 with Tween-40 using response surface methodology
复制标题
产碱菌胞外多糖韦兰胶生产的优化。
DOI：
10.1016/j.carbpol.2011.09.026
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发表时间：
2012-01-15
影响因子：
11.2
通讯作者：
Ouyang, Pingkai
中科院分区：
文献类型：
--
作者：
Li, Hui;Xu, Hong;Ouyang, Pingkai
研究了添加剂对Alcaligenes sp. CGMCC2428发酵生产welan gum的影响。Tween-40是提高威兰胶产量的最佳添加剂，威兰胶的流变性能优于不添加添加剂的对照发酵。采用响应面法对摇瓶培养中发酵威兰胶的培养条件进行优化，包括吐温-40浓度、pH和培养温度。确定了最佳工艺条件为：吐温-40浓度0.94 g/l， pH为6.9，温度为29.6℃，相应的实验胶浓度为23.62 +/- 0.60 g/l，与预测值（23.48 g/l）基本吻合。为了验证模型的充分性和准确性，还进行了验证实验。在7.51型生物反应器中发酵，发酵效果达到24.90 +/- 0.68 g/l。(C) 2011 Elsevier Ltd.版权所有。
The effect of additives on welan gum production produced by fermentation with Alcaligenes sp. CGMCC2428 was studied. Tween-40 was the best additive for improving welan gum production and welan gum displayed better rheological properties than that obtained by control fermentation without additives. Response surface methodology was employed to optimize the culture conditions for welan gum production in the shake flask culture, including Tween-40 concentration, pH and culture temperature. The optimal conditions were determined as follows: Tween-40 concentration 0.94 g/l, pH 6.9 and temperature 29.6 degrees C. The corresponding experimental concentration of welan gum was 23.62 +/- 0.60 g/l, which was agreed closely with the predicted value (23.48 g/l). Validation experiments were also carried out to prove the adequacy and the accuracy of the model obtained. The welan gum fermentation in a 7.51 bioreactor reached 24.90 +/- 0.68 g/l. (C) 2011 Elsevier Ltd. All rights reserved.