A new (R)-hydroxynitrile lyase from Prunus mume:: asymmetric synthesis of cyanohydrins
A new (R)-hydroxynitrile lyase from Prunus mume:: asymmetric synthesis of cyanohydrins
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DOI：
10.1016/j.tet.2005.08.105
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发表时间：
2005-11-14
期刊：
影响因子：
2.1
通讯作者：
Asano, Y
中科院分区：
文献类型：
--
作者：
Nanda, S;Kato, Y;Asano, Y
从日本杏(Prunus Mume)种子中分离到一种新的羟腈裂解酶(HNL)。该酶与从其他李属植物中分离到的HNL具有相似的性质，是含有FAD酶的。它接受大量非天然底物(苯甲醛及其变种)，用于HCN的加成，以优异的光学和化学产率产生相应的氰醇。在pH=4.5的缓冲溶液中，该酶能促进KCN与苯甲醛的加成反应，建立了一种新的基于高效液相色谱的对映体分析技术。(C)2005爱思唯尔有限公司。保留所有权利。
A new hydroxynitrile lyase (HNL) was isolated from the seed of Japanese apricot (Prunus mume). The enzyme has similar properties with HNL isolated from other Prunus species and is FAD containing enzyme. It accepts a large number of unnatural substrates (benzaldehyde and its variant) for the addition of HCN to produce the corresponding cyanohydrins in excellent optical and chemical yields. A new HPLC based enantioselective assay technique was developed for the enzyme, which promotes the addition of KCN to benzaldehyde in a buffered solution (pH = 4.5). (c) 2005 Elsevier Ltd. All rights reserved.