Genome-wide survey and expression profiling of heat shock proteins and heat shock factors revealed overlapped and stress specific response under abiotic stresses in rice
Genome-wide survey and expression profiling of heat shock proteins and heat shock factors revealed overlapped and stress specific response under abiotic stresses in rice
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DOI：
10.1016/j.plantsci.2009.01.016
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发表时间：
2009-04-01
期刊：
影响因子：
5.2
通讯作者：
Han, Bin
中科院分区：
文献类型：
--
作者：
Hu, Wenhuo;Hu, Guocheng;Han, Bin
热休克蛋白(HSPs)是一类分子伴侣，参与蛋白质的折叠和组装，蛋白质在细胞内的定位和分泌，以及错误折叠和截断蛋白质的降解。热休克因子(Hsf)是热休克蛋白的转录激活因子。据报道，热休克蛋白和热休克蛋白广泛参与响应各种非生物胁迫，如热、干旱、盐度和寒冷。为了阐明水稻HSP和HSF基因的功能和调控，我们研究了热胁迫下水稻幼苗的整体表达谱，并将我们的结果与以往水稻在低温、干旱和盐胁迫下的数据进行了比较。比较表明，虽然大多数Hsf和HSPs在不同的胁迫下具有高度相似和重叠的反应和调控模式，但其中一些基因对不同的胁迫表现出显著的特异性反应。我们还发现，热响应基因图谱与冷/干旱/盐胁迫下的基因谱有很大不同，干旱处理比拟南芥更有效地上调HSF的表达。总体而言，我们的数据表明，HSPs和Hsf可能是不同应激信号转导网络之间相互作用的重要元素。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
Heat shock proteins (Hsps) are molecular chaperons, which function in protein folding and assembly, protein intracellular localization and secretion, and degradation of misfolded and truncated proteins. Heat shock factors (Hsfs) are the transcriptional activators of Hsps. It has been reported that Hsps and Hsfs are widely involved in response to various abiotic stresses such as heat, drought, salinity and cold. To elucidate the function and regulation of rice Hsp, and Hsf genes, we examined a global expression profiling with heat stressed rice seedling, and then compared our results with the previous rice data undercold, drought and salt stresses. The comparison revealed that, while most Hsfs and Hsps had highly similar and overlapped response and regulation patterns under different stresses, some of those genes showed significantly specific response to distinct stress. We also found that heat-responsive gene profiling differed largely from those under cold/drought/salt stresses, and that drought treatment was more effective to up-regulate Hsf expression in rice than in Arabidopsis. Overall, our data suggests that Hsps and Hsfs might be important elements in cross-talk of different stress signal transduction networks. (C) 2009 Elsevier Ireland Ltd. All rights reserved.