High-resolution magic angle spinning NMR studies for metabolic characterization of Arabidopsis thaliana mutants with enhanced growth characteristics
High-resolution magic angle spinning NMR studies for metabolic characterization of Arabidopsis thaliana mutants with enhanced growth characteristics
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DOI：
10.1371/journal.pone.0209695
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发表时间：
2018-12-31
期刊：
影响因子：
3.7
通讯作者：
Alia, A.
中科院分区：
文献类型：
--
作者：
Augustijn, Dieuwertje;van Tol, Niels;Alia, A.
开发产生更多生物质的智能作物以满足对植物生物质日益增长的需求，在过去几十年中一直是一个活跃的研究领域。我们研究了两个拟南芥突变体的代谢变化，增强了生长特性，以前获得的植物株系表达人工转录因子的集合。使用高分辨率魔角旋转（HR-MAS）NMR直接从完整的拟南芥叶片中获得代谢谱。多变量分析显示突变体和野生型Col-0之间代谢物水平的显著改变。有趣的是，在快速生长的突变体中减少的大多数代谢物通常参与防御压力。这些结果表明，在表型工程突变体的生长防御权衡。我们的研究结果进一步证实了植物生长可以通过抑制防御途径来增强的想法。
Developing smart crops which yield more biomass to meet the increasing demand for plant biomass has been an active area of research in last few decades. We investigated metabolic alterations in two Arabidopsis thaliana mutants with enhanced growth characteristics that were previously obtained from a collection of plant lines expressing artificial transcription factors. The metabolic profiles were obtained directly from intact Arabidopsis leaves using high-resolution magic angle spinning (HR-MAS) NMR. Multivariate analysis showed significant alteration of metabolite levels between the mutants and the wild-type Col-0. Interestingly, most of the metabolites that were reduced in the faster-growing mutants are generally involved in the defence against stress. These results suggest a growth-defence trade-off in the phenotypically engineered mutants. Our results further corroborate the idea that plant growth can be enhanced by suppressing defence pathways.