A major thyroid hormone response element in the third intron of the rat growth hormone gene.
A major thyroid hormone response element in the third intron of the rat growth hormone gene.
复制标题
大鼠生长激素基因第三内含子中的主要甲状腺激素反应元件。
作者：
J. Sap;L. Magistris;H. Stunnenberg;B. Vennström
大鼠生长激素（RGH）基因构成了甲状腺激素直接调节转录的有据可查的模型系统。为了分析其与RGH基因序列的相互作用，我们使用牛痘病毒表达系统过量生产了甲状腺激素受体α（c-erbA）蛋白。表达的蛋白质以预期亲和力结合T3和DNA-纤维素，发现受体蛋白的主要结合位点位于RGH基因的第三内含子中。该网站显示显着更高的亲和力的受体蛋白比以前描述的甲状腺激素反应元件（TRE）在该基因的启动子，并赋予更强的激素反应性在体内的异源启动子。这些数据表明，这种新的TRE在甲状腺激素调节大鼠生长激素基因表达中起着重要作用。
The rat growth hormone (RGH) gene constitutes a well‐documented model system for the direct regulation of transcription by thyroid hormones. In order to analyse its interaction with sequences in the RGH gene, we have overproduced the thyroid hormone receptor‐alpha (c‐erbA) protein using a vaccinia virus expression system. The expressed protein bound T3 and DNA‐cellulose with expected affinities, and the major binding site for the receptor protein was found to be located in the third intron of the RGH gene. This site displayed significantly higher affinity for the receptor protein than a previously described thyroid hormone response element (TRE) in the promoter of this gene, and also conferred stronger hormone responsiveness in vivo to a heterologous promoter. The data suggest that this novel TRE plays a major role in the regulation of rat growth hormone gene expression by thyroid hormones.