Influence of surface coatings of poly(d,l-lactide-co-glycolide) particles on HepG2 cell behavior and particle fate.
Influence of surface coatings of poly(d,l-lactide-co-glycolide) particles on HepG2 cell behavior and particle fate.
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DOI：
10.1116/1.4894531
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发表时间：
2014-09
期刊：
影响因子：
2.1
通讯作者：
D. Yu;Yuying Zhang;Guangyang Zou;Xiaojing Cui;Z. Mao;Changyou Gao
中科院分区：
文献类型：
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作者：
D. Yu;Yuying Zhang;Guangyang Zou;Xiaojing Cui;Z. Mao;Changyou Gao
本研究主要探讨了不同表面涂层的聚丙交酯-乙交酯共聚物（PLGA）颗粒在细胞摄取后的细胞内命运及其对人肝癌细胞（HepG 2细胞）功能的影响。制备了聚乙烯亚胺（PEI）和牛血清白蛋白（BSA）包覆的PLGA微球，粒径约为400 nm，但表面化学性质不同。PLGA颗粒的细胞内分布也在很大程度上取决于其表面涂层。PLGA-PEI颗粒通过胞吐作用以比PLGA-BSA颗粒更慢的速率从细胞中除去。通常，两种类型的PLGA颗粒的摄取对细胞活力和细胞周期没有引起明显的阻抗，但是PLGA-PEI颗粒的摄取确实对细胞功能具有一定的影响，例如细胞内活性氧水平、细胞骨架组织、细胞迁移和甘油三酯分泌水平。
This study is focused on the intracellular fate of poly(d,l-lactide-co-glycolide) (PLGA) particles with different surface coatings after cellular uptake, and their influence on the functions of human liver cancer cells (HepG2 cells). The PLGA particles coated with polyethyleneimine (PEI) and bovine serum albumin (BSA) with a similar diameter of ∼400 nm but different surface chemistry were prepared. The intracellular distribution of the PLGA particles was also largely dependent on their surface coatings. The PLGA-PEI particles were removed from cells by exocytosis with a slower rate compared to the PLGA-BSA particles. In general, uptake of both types of the PLGA particles did not cause apparent impedance on cell viability and cell cycle, but uptake of the PLGA-PEI particles did have certain influence on cell functions such as intracellular level of reactive oxygen species, cytoskeleton organization, cell migration, and secretion levels of triglyceride.