The core populations and co-occurrence patterns of prokaryotic communities in household biogas digesters.
The core populations and co-occurrence patterns of prokaryotic communities in household biogas digesters.
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户用沼气池原核生物群落核心种群及共现模式
DOI：
10.1186/s13068-015-0339-3
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发表时间：
2015
影响因子：
6.3
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Rui J;Li J;Zhang S;Yan X;Wang Y;Li X
在发展中国家的农村地区，家用沼气池被广泛用于收集能源。了解核心原核生物群落，它们的共存模式，以及它们与环境因素的关系，对于有效管理这些小规模厌氧消化系统是很重要的。在这项研究中，43户沼气池收集了8个省在中国。使用454焦磷酸测序的16 S rRNA genes.Results14个核心属和10个核心OTU被确定在户用沼气池中的原生生物群落进行了调查。它们主要隶属于厚壁菌门、增效菌门、放线菌门、绿杆菌门和螺旋体门。核心原核菌属主要由梭菌属、梭菌属XI、互养单胞菌属、梭菌属、沉降杆菌属和粪杆菌属组成。43个样地的原生动物群落明显分为两个类群。聚类Ⅰ以梭菌属为主，聚类Ⅱ以螺旋体属、类杆菌目、梭菌属为主，并有丰富的互养菌和产甲烷菌。NH 4 +-N和COD对聚类I中原核生物群落的组装有显著贡献，而NH 4 +-N、pH和磷酸盐对聚类II有显著贡献。基于相关性的网络分析表明，沼气池中的原核生物群落主要由一些功能模块组成。簇I由产乙酸甲烷模块和梭菌驱动的初级发酵模块主导，而簇II的网络由产氢和产乙酸甲烷模块和多组驱动（螺旋体、拟杆菌目和梭菌）初级发酵模块主导。第二类的网络是更复杂和功能redundant. ConclusionsPropestrians社区确定在户用沼气池变化显着，受环境因素，如NH 4 +-N，pH值，和COD。然而，核心原核生物群落存在，其中大多数也是优势种群。世界性OTU倾向于共存。沼气池中的原生动物群落由一些功能模块组成。原核生物群落的模块化结构具有功能冗余性，在厌氧消化过程中增强了对环境胁迫的抵抗力，维持了消化效率。
BackgroundHousehold biogas digesters are widely used to harvest energy in rural areas of developing countries. Understanding core prokaryotic communities, their co-occurrence patterns, and their relationships to environmental factors is important to manage these small-scale anaerobic digestion systems effectively. In this study, 43 household biogas digesters were collected across eight provinces in China. Prokaryotic communities were investigated using 454 pyrosequencing of 16S rRNA genes.ResultsFourteen core genera and ten core OTUs were identified in household biogas digesters. They were mainly affiliated with the phylum Firmicutes, Synergistetes, Actinobacteria, Chloroflexi, and Spirochaetes. Core prokaryotic genera were mainly composed ofClostridium,ClostridiumXI,Syntrophomonas,Cloacibacillus,Sedimentibacter,andTuricibacter. Prokaryotic communities in the 43 samples were clearly divided into two clusters. Cluster I was dominated byClostridium, while Cluster II was dominated by members of Spirochaetes, Bacteroidales, Clostridia, and abundant syntrophs and methanogens. NH4+-N and COD contributed significantly to the assembly of the prokaryotic community in Cluster I, while NH4+-N, pH, and phosphate contributed significantly to Cluster II. Correlation-based network analysis showed that the prokaryotic communities in the biogas digesters were dominated by some functional modules. Cluster I was dominated by acetotrophic methanogenic modules and theClostridium-driven primary fermentation module, while the network of Cluster II was dominated by hydrogenotrophic and acetogenic methanogenesis modules and multi-group-driven (Spirochaetes, Bacteroidales, and Clostridia) primary fermentation modules. The network of Cluster II was more complex and functionally redundant.ConclusionsProkaryotic communities identified in the household biogas digesters varied significantly and were affected by environmental factors, such as NH4+-N, pH, and COD. However, core prokaryotic communities existed, and most of them were also dominant populations. Cosmopolitan OTUs tended to co-occur. Prokaryotic communities in biogas digesters were well organized by some functional modules. The modular structure of the prokaryotic community, which has functional redundancy, enhances the resistance against environmental stress and maintains digestion efficiency in the anaerobic digestion process.